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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Ready biodegradable, 63.5 % biodegradation after 28 days, OECD 301 D, EPA OPTS 835.3110, and EU Method C.4-E, Sipos (2013a).

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

In the key study (Sipos, 2013a) the ready biodegradability of the test material was determined in a Closed Bottle Test over 28 days using sewage obtained from a domestic sewage plant. The study was performed under GLP conditions and in accordance with the standardised guidelines OECD 301 D, EPA OPTS 835.3110, and EU Method C.4-E.

Under the conditions of the test, the test material was determined to have a percentage biodegradation of 63.5 % after 28 days based on the measured COD of the test material. According to the test guidelines the pass level for ready biodegradability, 60 % of COD and this level has to be reached in a 10-day window within the 28-day period of the test. However taking into account that the test material was sufficiently degraded to a mean of 64.3 % after 14 days and according to the test guideline the value obtained in a 14-day window is acceptable in the used method, therefore the test material can be considered to be ready biodegradable.

In the toxicity control containing both, the test material and the reference substance Sodium benzoate, a mean of 45.0 % biodegradation was noted within 14 days and 47.9 % biodegradation after 28 days of incubation. According to the test guidelines the test material can be assumed to be not inhibitory at the applied concentration level of 3.0 mg/L on the activated sludge microorganisms because degradation was > 25 % within 14 days.