Lactofen

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Principles of method if other than guideline:

The acute inhalation toxicity of PPG-844 was assessed by exposing 10 rats (5 males and 5 females), for a period of 4 hours, to an atmosphere containing a respirable aerosol of the test substance. The animals were observed for mortality, clinical signs, food and water consumption. At the end of the 14-day observation period the rats were subjected to a detailed macroscopic examination.

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

(2-ethoxy-1-methyl-2-oxoethyl)-5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate

EC Number:

616-466-9

Cas Number:

77501-63-4

Molecular formula:

C19H15ClF3NO7

IUPAC Name:

(2-ethoxy-1-methyl-2-oxoethyl)-5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate

Test material form:

liquid: viscous

Details on test material:

The test substance was a brown viscous liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

head only

Vehicle:

other: 10% acetone

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

Group 1 (control)
Group 2 (vehicle control)
Group 3 (PPG-844)

Atmosphere concentration of PPG-844:
By gravimetric analysis: 3.6 g/m³
By HPLC analysis: 3.4 g/m³

No. of animals per sex per dose:

5 mala and 5 female animals per group

Control animals:

yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 3.4 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Any other information on results incl. tables

Mortality: There were no deaths related to exposure to PPG-844

Clinical signs: a) during exposure: no adverse signs were visible b) brown staining of the fur and poor groming was noted in the rats exposed to PPG-844. These signs persisted for 2 days (males) or 11 days (females).

Body weight: no effect

Food consumption: reduced for all groups on the day following exposure

Water consumption: markedly increased water consumption for 5 days (males) or 3 days (females)

Macroscopic pathology: no abnormalities were seen in any rat

Applicant's summary and conclusion

Executive summary:

The acute inhalation toxicity of PPG-844 was assessed by exposing 10 rats (5 males and 5 females), for a period of 4 hours, to an atmosphere containing a respirable aerosol of the test substance. The animals were observed for mortality, clinical signs, food and water consumption. At the end of the 14-day observation period the rats were subjected to a detailed macroscopic examination.

LC50 (4 hours) for PPG-844: In excess of 3.4 g/m³ which was the highest attainable aerosol concentration.