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EC number: 240-383-3
CAS number: 16291-96-6
An amorphous form of carbon produced by partially burning or oxidizing wood or other organic matter.
DPM and SI:
Test item concentration % (w/w)
number of lymph nodes
DPM per lymph nodeb)
BG= Background (1 ml 5% trichloroacetic acid) in duplicate
1 = Control Group
2-4= Test Groups
S.I.= Stimulation Index
a) = The mean value was taken from the figures
BG I and BG II
b) = Since the lymph nodes of the animals of
a dose group were pooled, DPM/node was determined by dividing the
measured value by the number of lymph nodes pooled
The estimated concentration of the test item required to
produce a 3-fold increase in draining lymph node cell
proliferative activity (termed the EC3 value) can be calculated
according to the equation [EC3=(a-c) [(3-d)/(b-d)] + c].
Here, (a, b) and (c, d) are respectively the co-ordinates of the
two pair of data lying immediately above and below the S.I. value
of 3 on the local lymph node assay dose response plot.
The EC3 value could not be calculated, since all S.I.´s are
No deaths occurred during the study period.
No symptoms of local toxicity at the ears of the animals and
no systemic findings were observed during the study period.
The body weight of the animals, recorded prior to the first
application and prior to treatment with 3HTdR, was within the
range commonly recorded for animals of this strain and age.
In order to study a
possible contact allergenic potential of Charcoal (Probe 1: C-Fix
= 73.3%), three groups each of four female mice were treated daily
with the test item at concentrations of 2.5, 5, and 10% (w/w) in
propylene glycol by topical application to the dorsum of each ear
(left and right) for three consecutive days. A control group of
four mice was treated with the vehicle (propylene glycol) only.
Five days after the first topical application the mice were
injected intravenously into a tail vein with 3HTdR. Approximately
5 h after intravenous injection, the mice were sacrificed, the
draining auricular lymph nodes excised and pooled per group.
Single cell suspensions of lymph node cells were prepared from
pooled lymph nodes, which were subsequently washed and incubated
with trichloroacetic acid overnight. The proliferative capacity of
pooled lymph node cells was determined by the incorporation of
3HTdR measured in a beta-scintillation counter.
All treated animals
survived the scheduled study period and no signs of toxicity were
A test item is
regarded as a sensitiser in the LLNA if the exposure to one or
more test concentration resulted in 3-fold or greater increase in
incorporation of 3HTdR compared with concurrent controls, as
indicated by the Stimulation Index (S.I.). The estimated
concentration of test item required to produce a S.I. of 3 is
referred to as the EC3 value. In this study Stimulation Indices of
0.65, 0.72, and 1.11 were determined with the test item at
concentrations of 2.5, 5, and 10%, respectively, in propylene
glycol. The EC3 value could not be calculated, since none of the
tested concentrations induced an S.I. greater than 3.
The test item,
charcoal (Probe 1: C-Fix = 73.3%) was not a skin sensitiser under
the test conditions of this study.
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