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EC number: 240-383-3 | CAS number: 16291-96-6 An amorphous form of carbon produced by partially burning or oxidizing wood or other organic matter.
Comparison of formazan production:
For the test item and the positive control the following percentage values of formazan production were calculated in comparison to the negative control:
% Formazan Production
Charcoal (Probe1: C-Fix=73.3%)
% Formazan production (Tissue 1)
% Formazan production (Tissue 2)
% Formazan production (Tissue 3)
% Formazan production Mean
This in-vitro study human skin model test (EU method B.46) was performed in order to evaluate the potential of charcoal (Probe 1: C-Fix = 73.3%) to evoke skin irritation in a human-skin-model. Charcoal was spread on the surface of three sections of an EpiDermTMtissue and incubated for 60 min. The negative control consisting of Dulbecco’s phosphate buffered saline was applied to another three tissue sections. As positive control a SDS-solution (50 g/L) was used for three other tissue sections. After rinsing and two incubation steps of altogether 42 h an enzyme assay to determine cell viability was performed. The tissues were incubated with MTT-reagent for 3 h. The tissues were washed with PBS, dried and transferred to a 24 -well-plate. After incubating with isopropanol at room temperature over night, the developed formazan concentration was measured in a spectral photometer at 570 nm.
The mean percentage values of formazan production were calculated in comparison to the negative control. The relative absorbance values were reduced to 70.4% after the treatment with charcoal. This value is above the threshold for irritation (50%). The positive control showed a reduction of formazan production of 7.8%.
In conclusion, it can be stated that the test item charcoal (Probe 1: C-Fix = 73.3%) is considered as non skin irritant under the experimental conditions used in this study.
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