2-benzyl-2-dimethylamino-4'-morpholinobutyrophenone

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-benzyl-2-dimethylamino-4'-morpholinobutyrophenone

EC Number:

404-360-3

EC Name:

2-benzyl-2-dimethylamino-4'-morpholinobutyrophenone

Cas Number:

119313-12-1

Molecular formula:

C23 H30 N2 O2

IUPAC Name:

2-benzyl-2-(dimethylamino)-1-[4-(morpholin-4-yl)phenyl]butan-1-one

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Concentrations: 200 and 100 mg/L (nominal)
Sampling method: Filtration of test medium samples (10 mL) taken at the start of the tests, 24, and 72 hours of exposure

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Hardness:

24 mg CaCO3/L

Test temperature:

22.5 - 24 °C

pH:

8.0 - 8.4

Nominal and measured concentrations:

Water accomodated fractions were prepared with a loading rate of 100 and 200 mg/L. Measured concentrations were at most 2 mg/L. The test substance is rapidly degraded by photolysis.

Details on test conditions:

TEST CONCENTRATIONS
- Rough filtrates (ca. 5 µm) of supersaturated solutions of 100 and 200 mg/L stirred for ca. 4 days.
- Justification for using less concentrations than requested by guideline: The substance is insoluble in water (< 0.006 g/L at 20 °C).
- Range finding study: A measured concentration of 4.7 mg/L was found in the sample taken from the filtrate. Instead of testing dilutions containing 0.1 to 100 % of the filtrate, test solutions contained 0.05 to 50 % of the filtrate yielding a test substance concentration range from 0.002 to 2.35 mg/L. No inhibition of algal growth was observed at any of the test concentrations.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 2 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: No acute toxic effects within the range of solubility

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 2 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: No acute toxic effects within the range of solubility

Details on results:

Exponential growth in the control (for algal test): yes
Observation of abnormalities (for algal test): None

Results with reference substance (positive control):

Results with reference substance valid? Yes
EC50: 0.98 mg/L (growth inhibition); 1.1 mg/L (growth rate reduction)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No acute toxic effects within the range of solubility. The test substance is with high probability acutely not toxic to aquatic algae.