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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014
Report date:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Reaction mass of 3-methyl-1-octylpyridinium chloride and 4-methyl-1-octylpyridinium chloride
EC Number:
941-484-1
Molecular formula:
not possible
IUPAC Name:
Reaction mass of 3-methyl-1-octylpyridinium chloride and 4-methyl-1-octylpyridinium chloride
Test material form:
liquid: viscous

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Sampling method:
All concentration levels and the control were analytically verified via the test item LC-MS/MS at the start (0 h) and at the end of the exposure (48 h).
At the start of the exposure (0 h), sampling was carried out after preparation of the test concentrations. At the end of the exposure (48 h), samples were taken directly from the test vessels.

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Agitation
- Controls: Dilution water without test item tested under the same conditions as the test groups
- Evidence of undissolved material: test item is completely dissolved

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Method of breeding: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20  2°C, in an incubator, 16 h illumination, light intensity of max. 20 µE x m-2 x s-1.
- Feeding during test
The culture daphnids are fed at least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus, with an algae cell density of > 10^6 cells/mL. The algae are cultured at the test facility.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
total hardness: 260 [mg CaCO3/L]
Test temperature:
18 - 22°C, constant within ± 1°C
pH:
7.03 - 7.15
Nominal and measured concentrations:
The measured test item concentrations were all within ± 20 % of the nominal concentrations. This indicates that the test item concentrations were successfully maintained for the duration of the test.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all glass test aquaria of 50 mL volume filled with a20 mL test medium
- Aeration: no
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: double distilled water
- Total Hardness [mg CaCO3/L]: 260
- Conductivity: 662 µS/cm
- pH-Value: 7.63
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 h light/dark cycle
- Light intensity: Diffuse light, light intensity of max. 20 µE x m-2 x s-1

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilisation was monitored one and two days after exposure

TEST CONCENTRATIONS
- Spacing factor for test concentrations: The measured test item concentrations were all within ± 20 % of the nominal concentrations.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate p.a. (SIGMA)

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.446 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 0.421 – 0.472 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
0.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: all daphnids were mobile in the control during the 2 days of exposure.
- Any observations that might cause a difference between measured and nominal values: the test substance was soluble in medium, the test concentrations were present as a solution.
Results with reference substance (positive control):
- Results with reference substance valid? yes
- EC50/LC50: EC50 (24 h) = 2.01 mg/L

Any other information on results incl. tables

PYRIDINBASE
Bulk SPECIAL

Nominal
test item
concentration
[mg/L]


IMMOBILISATION [%]
24 h 48 h
Replicates Replicates
1. 2. 3. 4. MV 1. 2. 3. 4. MV
2.00 100 100 100 100 100 100 100 100 100 100
1.00 80 40 40 60 55 100 100 100 100 100
0.500 40 40 0 20 25 100 60 60 60 70
0.250 0 0 0 0 0 0 0 0 0 0
0.125 0 0 0 0 0 0 0 0 0 0
Control 0 0 0 0 0 0 0 0 0 0

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the nominal concentrations of the test item PYRIDINBASE Bulk SPECIAL, the 48 h-EC50 for Daphnia magna was 0.446 mg/L (95 % confidence limits: 0.421 – 0.472 mg/L). The NOEC after 48 h was 0.250 mg/L. The LOEC after 48 h was 0.500 mg/L.