Registration Dossier
Registration Dossier
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EC number: 941-484-1 | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of 3-methyl-1-octylpyridinium chloride and 4-methyl-1-octylpyridinium chloride
- EC Number:
- 941-484-1
- Molecular formula:
- not possible
- IUPAC Name:
- Reaction mass of 3-methyl-1-octylpyridinium chloride and 4-methyl-1-octylpyridinium chloride
- Test material form:
- liquid: viscous
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Colony forming units (CFU) in the test vessel: Approx. 1.98 x 10^7 CFU/L
- Duration of test (contact time):
- 28 d
- Details on study design:
- TEST CONDITIONS
- Test temperature: Nominal 22 ± 2 °C, actually measured 20.5 – 23.5 °C
- pH: 7.64- 7.83
- pH adjusted: no
TEST SYSTEM
- Culturing apparatus: 5000 mL glass vessels
- Number of culture flasks/concentration: 1
SAMPLING
- Sampling frequency: 1, 4, 6, 8, 11, 14, 18, 21, 25, 28 and 29 days
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Batch number: BCBL4472V Fluka
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 1 d
- Remarks on result:
- other: Replicate 1
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1
- Sampling time:
- 6 d
- Remarks on result:
- other: Replicate 1
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 3
- Sampling time:
- 11 d
- Remarks on result:
- other: Replicate 1
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 5
- Sampling time:
- 21 d
- Remarks on result:
- other: Replicate 1
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 6
- Sampling time:
- 28 d
- Remarks on result:
- other: Replicate 1
BOD5 / COD results
- Results with reference substance:
- Kinetic of control substance:
67% after 6 days, 83% after 11 days, 88% after 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- Under present conditions the biodegradability of the test item was 6% of the initial amount after 28 days.
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