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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study without restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-chlorobenzaldehyde
EC Number:
201-956-3
EC Name:
2-chlorobenzaldehyde
Cas Number:
89-98-5
Molecular formula:
C7H5ClO
IUPAC Name:
2-chlorobenzaldehyde
Details on test material:
- Name of test material (as cited in study report): o-chlorobenzaldehyde
- Physical state: liquid
- Analytical purity: 99.42 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: green algae
- Strain: SAG 61.81
- Source (laboratory, culture collection): Algae Collection University of Göttingen, Germany, cultivated at the performing institute.
- Method of cultivation: Algae were transferred from agar bevels to the fresh medium (for Pseudokirchneriella subcapitata AAP medium is recommended, Appendix 2) in 250 mL Erlenmeyer flask and incubated in 21 – 24 C in constant illumination (pre-culture) [1], [SOP/W/65]. The algae culture was renewed (inoculated to a new medium) twice a week in sterile conditions [SOP/W/56, SOP/W/70].
A pre-culture with cell density of 1 x 104 per mL was renewed three days before the definitive test. The pre-culture was used for inoculation of the test concentrations and the control.

ACCLIMATION
- Culturing media and conditions (same as test or not): yes
- Any deformed or abnormal cells observed: no

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Nominal and measured concentrations:
Nominal: 100, 40, 16, 6.4, 2.56 and 1.02 mg/L
Measured: 94.9, 90.3, 68.7, 2.6, 1.9% of nominal concentration and below the LoD value (i.e. 0.005 mg/L)
Details on test conditions:
TEST SYSTEM
- Test vessel: glass Erlenmeyer flasks 250 mL
- Type (delete if not applicable): closed with air permeable stoppers
- Material, size, headspace, fill volume: 100 mL
- Aeration: none
- Initial cells density: 10E+04 cells/mL
- Control end cells density:
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 2

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16:8 light:dark
- Light intensity and quality:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometric measurement

TEST CONCENTRATIONS
- Range finding study: yes
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
14.06 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 12.76 - 15.46 mg/L
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
2.11 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 1.66 - 2.59 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes

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