Disodium 4,4'-diaminostilbene-2,2'-disulphonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1986-11-26 to 1986-11-28

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study without anayltical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German industrial standard test guideline DIN 38412, part 11

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Disodium 4,4'-diaminostilbene-2,2'-disulphonate

EC Number:

230-847-3

EC Name:

Disodium 4,4'-diaminostilbene-2,2'-disulphonate

Cas Number:

7336-20-1

Molecular formula:

C14H14N2O6S2.2Na

IUPAC Name:

disodium 2,2'-ethene-1,2-diylbis(5-aminobenzenesulfonate)

Details on test material:

- Name of test material (as cited in study report): 4.4-Diamino-stilben-2.2-disulfonsaeure

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Source: Institute National de Recherche Chimique Appliquee, France
- Age at study initiation: 6-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.70 ± 0.50 mmol/L

Test temperature:

292 - 294 K

pH:

7.84 - 8.04

Dissolved oxygen:

9.17 - 9.64 mg/L

Nominal and measured concentrations:

Nominal test concentrations (mg/L): 3.905, 7.81, 15.6, 31.2, 62.5, 125.0, 250.0, 500.0, blank control

Details on test conditions:

TEST SYSTEM
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Photoperiod: No illumination

EFFECT PARAMETERS MEASURED: Mobility

TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 2

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

62.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

292.33 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 288.7 - 295.97 mg/l 95% CL

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: None

Any other information on results incl. tables

	Cumulative mobility after x hours
Nominal concentration (mg/l)		0 h	24 h	48 h
Control		20	20	20
3.905		20	20	20
7.81		20	20	20
15.6		20	19	19
31.2		20	20	19
62.5		20	20	19
125.0		20	19	12
250.0		20	17	10
500.0		20	17	9