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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(S)-2-Amino-6-propylamino-4,5,6,7-tetrahydrobenzothiazole
Cas Number:
104632-26-0
Molecular formula:
C10 H17 N3 S
IUPAC Name:
(S)-2-Amino-6-propylamino-4,5,6,7-tetrahydrobenzothiazole

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
>= 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
>= 70 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Conclusions:
Two valid experiments were performed.
The first experiment was performed using eight concentrations ranging from 40 to 1000 mg/L. Twenty daphnia were exposed to the test item for
48 hours in a static test system.After 24 and 48 hours, the immobilized daphnia were counted.
Four concentrations ranging from 40 to 160 mg/L showed toxicity between 0 and 100% immobilization after 48 hours of exposure. 5% of the
animals were immobilized in the control (criterion: <= 10%).
As the lowest concentration in the first experiment showed 15% immobilization, a second experiment was performed using the two concentrations
16 and 25 mg/L. None of the daphnia were immobilized in the control and the treatments.
At the beginning and at the end of each experiment, the content of the test item in the test solutions was determined using HPLC. The recovery after
48 hours was in a range of 100 to 109 % of the start concentration. The measured concentrations ranged from 96 to 111% of the nominal
concentration. Therefore, the determination of the results was based on the nominal concentrations.
The following results could be determined for the test item Pramipexole (species: Daphnia magna).
24h NOEC = 40 mg/L
48h NOEC = 25 mg/L
24h EC50i = 200 mg/L
48h EC50i = 70 mg/L

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