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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Ames test was realized according to OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report date:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
N-benzyl-2-bromo-3-methoxypropanamide
EC Number:
700-538-2
Cas Number:
705283-58-5
Molecular formula:
C11H14BrNO2
IUPAC Name:
N-benzyl-2-bromo-3-methoxypropanamide

Method

Target gene:
N-benzyl-2-bromo-3-methoxypropanamide was tested for a potential to induce reverse mutation at histidine or tryptophan loci in S.typhimurium
and E. coli.
Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Species / strain / cell type:
E. coli WP2 uvr A
Metabolic activation:
with and without
Metabolic activation system:
Rat liver S9 metabolic activation
Test concentrations with justification for top dose:
The concentrations 17, 50, 167, 500, 1667 and 5000µg/plate were chosen for experiments.
Controls
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: 2AAN : 2-Amino Anthracene for S9+ assays. NAN3 : Sodium Azide, ENNG : N-Ethyl-N-Nitro-N-Nitrozoguanidine, 2-NF : 2-Nitrofluorene and 9-AA : 9-Amino Acryline for S9- assays.
Details on test system and experimental conditions:
See study report attached.

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
not applicable
Positive controls validity:
valid
Species / strain:
E. coli WP2 uvr A
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
not applicable
Positive controls validity:
valid
Additional information on results:
Not mutagenic in strains of S.typhimurium and E.coli into DMSO up to 5000µg/plate.
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation