3-(3-tert-butyl-4-hydroxyphenyl)propionic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Jun. 27, 1988 to Aug. 8, 1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented, according to accepted guideline, with GLP-like Quality Assurance

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

Test performed as limit test with conc. 100 mg/l nominal. Highest vehicle concentration 950 mg/l; acclimatisation 11 days

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-(3-tert-butyl-4-hydroxyphenyl)propionic acid

EC Number:

403-920-4

EC Name:

3-(3-tert-butyl-4-hydroxyphenyl)propionic acid

Cas Number:

107551-67-7

Molecular formula:

C13H18O3

IUPAC Name:

3-(3-tert-butyl-4-hydroxyphenyl)propanoic acid

Details on test material:

- Physical state: beige crystalls
- Analytical purity: commercial grade
- Lot/batch No.: EN.116905.72
- Expiration date of the lot/batch: 11/92
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L.
- Sampling method: Samples for analysis were taken at the start and at the end of the exposure.
- Sample storage conditions before analysis: Not available.

Test solutions

Vehicle:

yes

Details on test solutions:

Stock solution: 5.0 g test substance were dissolved in and made up to 50 ml with DMF.
Highest vehicle concentration: 950 mg/L.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra-Fish.
- Source: West Aquarium / D-3422 Bad Lauterberg.
- Length at study initiation (length definition, mean, range and SD): 20 mm (16-25 mm).
- Weight at study initiation (mean and range, SD): 0.06 g (0.03-0.11 g).



ACCLIMATION
- Acclimation period: 11 days.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Not applicable.

Test conditions

Hardness:

188 mg CaC03/L.

Test temperature:

23 ± 1°C.

pH:

8.0-8.5.

Dissolved oxygen:

93-102%.

Salinity:

freshwater

Nominal and measured concentrations:

Nominal concentration: 100 mg/L . Refer to Table 1.

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria of 20 liters filled with 15 liters (36/22/25 cm).
- Type (delete if not applicable): open
- Aeration: None
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.04 g/l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water (carbon filter).
- Intervals of water quality measurement: oxygen, pH, temperature daily
OTHER TEST CONDITIONS
- Photoperiod: Fluorescent light, 16 hours daily.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Mortalities, swimming behaviour, loss of equilibrium, respiratory function, exophtalmus, and pigmentation observed 24, 48, 72 and 96 hours

Reference substance (positive control):

not required

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: None

Effect concentrations 2

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: None

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: None

Effect concentrations 4

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: None

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: None

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: None

Details on results:

Refer to Tables 2 and 3.

Results with reference substance (positive control):

Mortalities in blank (water) : 0%
Mortalities in vehicle (DMF) : 0%

Reported statistics and error estimates:

None.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Table 2. Mortalities

Concentration nominal mg/ L	Mortalities Number of dead fishes
	24 h	48 h	72 h	96 h
Blank	0	0	0	0
Vehicle	0	0	0	0
100	0	0	0	0
100	0	0	0	0

Table 3 Symptoms observed at the different test concentrations

Concentration nominal mg/ L	Swimming behaviour				Loss of equilibrium				Respiratory function				Exophtalmus				Pigmentation
	24	48	72	96h	24	48	72	96h	24	48	72	96h	24	48	72	96h	24	48	72	96h
Blank	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vehicle	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
100	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
100	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

Effect :

0: normal

1: slight

2: moderate

3: severe