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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
24 August 2007 - 21 September 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to relevant test guidelines, and in compliance with GLP.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: OECD Guidance Document 23 "Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures" (September 2000)
Deviations:
no
GLP compliance:
yes
Remarks:
The study report includes a statement of GLP compliance signed by the Study Director, and a statement of Quality Assurance, however no GLP certificate issued by an independant authority (such as an official GLP monitoring authority) was provided.
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L
- Sample storage conditions before analysis: Samples were not stored prior to analysis (analysed as samples taken).
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test material was added to dilution water and stirred to dissolve. No evidence of precipitation was seen at the start or end of the test.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Daphnia Magna
- Strain: Clone A
- Source: Young daphnids produced by parents which were cultured in the Kurume Laboratory were used. Daphnids [Daphnia magna (Clone A)] originally came from the University of Sheffield (Sheffield S10 2UQ, United Kingdom).
- Age at study initiation (mean and range, SD): less than 24 hours old
- Feeding during test Not fed

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
37.0 mg/L (nominal value from most recent sample, expressed in terms of CaCO3).
Test temperature:
20.1 - 20.3°C
pH:
7.6 - 7.8
Dissolved oxygen:
8.3 - 8.4 mg/L
Salinity:
Not measured
Nominal and measured concentrations:
101 mg/L at the start of the test; 95.1 mg/L at the end of the test.
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL glass beakers
- Type (delete if not applicable): closed (covered with a lid to prevent contamination by dust, and loss of test material through evaporation / volatilization).
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: No adjustment of pH
- Photoperiod: 16 hours light / 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilisation was recorded at 24 hours and 48 hours of the test.

TEST CONCENTRATIONS
Single concentration used at 100 mg/L (limit test).
A preliminary test was conducted at 100 mg/L, with 5 daphnids per vessel. As no immobility was seen in the preliminary test, the main study was conducted at the 100 mg/L limit.
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: No immobility was observed in the control group.

This study was conducted as a limit test to confirm that the test item has no effects on the test organisms at the maximum concentration (100 mg/L) on applied test guidelines. The measured concentrations of the test item in the test solution were maintained within ± 20% of the nominal concentration. At the end of the exposure, 3-methoxy-3-methylbutan-1-ol existed slightly in the test solution and acetic acid was not determined. It was decided that the test item and its hydrolysates exerted no effect on the test organisms because no adverse effect was detected in this study. In addition, the environmental conditions were also within the suitable range. It was determined, therefore, that this study complied with the applied test guidelines.

Validity criteria fulfilled:
yes
Conclusions:
No immobility and no abnormal response were observed in the exposure level during exposure. The 24-hour and 48-hour EC50s of the test item for Daphnia magna were >100 mg/L.

Description of key information

EC50, 48 hours for Daphnia Magna was found to be greater than 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information

An acute immobilisation test in Daphnia magna was conducted to assess the effect of the test material on aquatic invertebrates. The study was conducted according to OECD test guideline 202, and in compliance with GLP.

Four replicate vessels each containing five Daphnia were exposed to dilution water containing the test material at a limit nominal concentration of 100 mg/L. Daphnia were observed at 24 hours and 48 hours for immobilisation.

No immobilisation was seen in either the control level or in the test level at either 24 or 48 hours. On this basis the EC50 value for the test material was concluded to be greater than 100 mg/L.