Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 December 2009 to 23 February 2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted to GLP and in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do no effect the quality of the relevant results

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
Date of GLP inspection: 15/09/09 Date of Signature on GLP certificate: 26/11/09
Type of method:
HPLC estimation method
Media:
other: acetonitrile: reverse osmosis water (55:45 v/v)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Sponsor's identification : DVS005u (aka Weston 705)
Description : clear colourless viscous liquid
Batch number : MW9F23A901
Date received : 05 November 2009
Expiry date : 15 June 2010
Storage conditions : approximately 4°C in the dark, under nitrogen
Radiolabelling:
no

Study design

Test temperature:
40°C

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: HPLC
- Type: Agilent Technologies 1100, incorporating autosampler and workstation
- Type, material and dimension of analytical (guard) column: CN Optimal 5µ (150 x 4.6 mm)
- Detection system: UV

MOBILE PHASES
- Type: acetonitrile: reverse osmosis water (55:45 v/v)].
- Experiments with additives carried out on separate columns: no
- pH: 5.8
- Solutes for dissolving test and reference substances: All substances injected onto the HPLC were dissolved in the mobile phase.

DETERMINATION OF DEAD TIME
- Method: by means of homologous series

REFERENCE SUBSTANCES
- Identity: Acetanilide, Phenol, Atrazine, Isoproturon, Triadimenol, Linuron, Naphthalene, Endosulfan-diol, alpha-Endosulfan, Phenanthrene, Diclofop-methyl, DDT


DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 20 µl
- Quantity of reference substances: 10 µl
- Intervals of calibration:

REPETITIONS
- Number of determinations: Two

EVALUATION
- Calculation of capacity factors k': The capacity factor was determined by using the equation below:

k = (tr - to)/ to

where:
k = capacity factor
tr = retention time (min)
to = dead time (min)

- Calculation of retention times: Retention time measured in minutes

- Determination of the log Koc value: The adsorption coefficient was calculated using the equation below:

Log10 Koc = (Log10 k-A) / B

where:
Koc = adsorption coefficient
k = capacity factor
A = intercept of the calibration curve (see attachment 1 - calibration curve)
B = slope of the calibration curve (see attachment 1 - calibration curve)

Batch equilibrium or other method

Computational methods:
Not applicable

Results and discussion

Adsorption coefficientopen allclose all
Type:
Koc
Value:
> 4.27
Temp.:
40 °C
Type:
log Koc
Value:
> 5.63
Temp.:
40 °C

Results: HPLC method

Details on results (HPLC method):
- Retention times of reference substances used for calibration: - Please refer to Table in results section

- Details of fitted regression line (log k' vs. log Koc): Please refer to Attachment 1

- Graph of regression line attached: Yes

- Average retention data for test substance:
Injection 1: =12.641 minutes
Injection 2: = 12.632 minutes

Results: Batch equilibrium or other method

Transformation products:
no
Statistics:
none

Any other information on results incl. tables

Table 1

Standard

Retention Time (mins)

Mean Retention Time (mins)

Capacity Factor (k)

Log10k

Log10Koc

Injection 1

Injection 2

Acetanilide

2.273

2.273

2.273

0.294

-0.532

1.25

Phenol

2.413

2.414

2.414

0.374

-0.427

1.32

Atrazine

2.676

2.682

2.679

0.525

-0.280

1.81

Isoproturon

2.732

2.731

2.732

0.555

-0.256

1.86

Triadimenol

2.903

2.903

2.903

0.653

-0.185

2.40

Linuron

3.236

3.237

3.237

0.843

-7.44 x 10-2

2.59

Naphthalene

3.533

3.533

3.533

1.01

4.92 x 10-3

2.75

Endosulfan-diol

3.337

3.337

3.337

0.900

-4.59 x 10-2

3.02

a-Endosulfan

5.068

5.067

5.068

1.89

0.275

4.09

Phenanthrene

4.263

4.265

4.264

1.43

0.155

4.09

Diclofop-methyl

4.740

4.739

4.740

1.70

0.230

4.20

DDT

6.956

6.959

6.958

2.96

0.471

5.63

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The adsorption coefficient (Koc) of the test material has been determined to be greater than 4.27 x 105, log10 Koc >5.63.
Executive summary:

The determination was carried out using the HPLC screening method, Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001.

The adsorption coefficient (Koc) of the test material has been determined to be greater than 4.27 x 105, log10Koc >5.63.