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Short-term toxicity to aquatic invertebrates

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Description of key information

Cu(II)-IDHA_OECD202_Daphnia magna: EC50 (48h) = 37.72 mg/L

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
37.72 mg/L

Additional information

In a static acute toxicity study according to OECD Guideline 202 (Konfederak, 2011), young Daphnia magna individuals (< 24 h old) were exposed to the test item for 48 hours. Four replicates of each test concentration and control were tested with 5 daphnids per replicate (100 mL test volume). The test item Cu(II) IDHA was applied in five test concentrations: 6.25; 12.5; 25.0; 50.0 and 100 mg/L. Daphnids were observed for immobilization after 24 and 48 hours of exposure. The animals are considered immobile if they showed no ability to swim within 15 seconds after shaking the test vessel. The chemical analyses performed by validated method confirmed correct preparation of test concentrations. At test initiation the content of Cu(II) IDHA was 99.1 -104.9 % of nominal concentrations. At test termination the content of Cu(II) IDHA was 93.56 – 113.39 % of initial concentrations. The endpoint ECx values were determined based on nominal concentrations of Cu(II) IDHA. The test item caused immobilization of 30 % daphnids in test concentration 25 mg/L, 60 % in 50 mg/L and 100 % in 100 mg/L. As final results, the EC50 (48 h) is 37.72 mg/L and EC100 (48 h) value is 100 mg/L - based on nominal concentrations of Cu(II) IDHA. The NOEC/EC0 value after 48 h amounts to 12.5 mg/L. The LOEC value after 48 h is 25 mg/L.