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Short-term toxicity to fish

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Description of key information

Cu(II)-IDHA_OECD203_Oncorhynchus mykiss: LC50 (96h) > 100 mg/L

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

The acute toxicity of Cu(II)-IDHA towards Rainbow trout was investigated according to OECD Guideline 203 in compliance with GLP (Świerkot, 2011). After performing a preliminary test, Oncorhynchus mykiss was exposed in a static freshwater test to the test item at nominal concentrations of 0 (control), 10, 18, 32, 56 and 100.0 mg/L.

The exposure took place in glass aquaria with a capacity of 10 L. Seven fish were introduced into each aquarium. The fish were observed for mortality and intoxication symptoms after 3, 6, 24, 48, 72, and 96 hours exposure. In all the test concentrations and in control neither mortality nor symptoms were observed during the test. Chemical analyses were performed using LC-UV/VIS. Samples collected at the exposure initiation (t0) and at the exposure termination (t96) were analysed. The concentration of Cu(II)-IDHA in samples collected at the exposure initiation (t0) was between 88 and 120 % of the nominal concentration. The average concentration of Cu(II)-IDHA in samples collected at the exposure termination (t96) was between 89 and 104 % of the initial concentration. Since the concentration of the test item in the solution was higher than 80% of the initial concentration after 96 hours, the LCx values were calculated on the basis of the nominal concentration.

Median concentration causing 50 % mortality of the fish population during 96 hours exposure time (LC50 (96 h)) is higher than 100 mg/L. The LC0 (96 h) is equal or higher than 100 mg/L and LC100 (96 h) is higher than 100 mg/L of nominal test item concentration. Due to the absence of mortality and any intoxication symptoms at the highest concentration tested, the NOEC (96 h) value can be indicated as ≥ 100 mg/L based on the nominal concentration.