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A reproduction study with the earthworm Eisenia fetida in an artificial soil substrate was performed to assess any possible effect of FR-245 on soil macroorganisms. No mortality or other effects were noted in the adult earthworms and no effect on reproduction was oberved. NOEC for both growth and reporoduction were >1000mg/kg dw soil.

In order to determine the effect of the test substance on the seedling emergence and growth, six species of terrestrial non-target higher plants were tested. Results: A soil-incorporated application of FR-245 at nominal concentrations ranging up to 1000

mg/kg dry soil resulted in slight treatment related effects, but no reductions greater than 50% relative to the

control were observed on any test endpoint for any species. The EC50 for all six species tested was greater than 1000mg/kgdry soil.

NOEC forAllium cepa and Cucumis sativuswas greater than 1000mg/kgdry soil. The NOEC for Lolium perenne

and Brassica albawas greater than 333mg/kgdry soil. The NOEC for Lactuca sativawas greater than 37

mg/kgdry soil. The most sensitive species was Lycopersicon esculentumwith an NOEC 12.3mg/kgdry soil.