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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1986-09-15 to 1986-09-19
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Remarks:
Study scientifically fully valid, but predating the implementation of GLP.
Qualifier:
equivalent or similar to
Guideline:
other: DIN 38 412, June 1982
Deviations:
no
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION: the test item was added to the test water without any pretreatment
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Golden orfe
- Strain: Leuciscus Idus L., golden variety
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 9.6 cm (range 8.1-11.9)
- Weight at study initiation: 9.6 g (range 7.4-17.1)

ACCLIMATION
- Acclimation period: 13 months
- Acclimation conditions: same as test conditions
- Type and amount of food: "FUKOSALM"-Growing Feed 1 and SSNIFF- Growing Feed F/B 50 Club, ad libitum
- Health during acclimation (any mortality observed): Mortality during the last two weeks of housing: about 0.3 %
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
2.5 mmol/L
Test temperature:
20 °C
pH:
about 7.9
Dissolved oxygen:
> 60 % of air saturation value
Nominal and measured concentrations:
Nominal: 0, 1000, 5000 and 10000 mg/L
Measured: not determined
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquarium
- Type: open
- Material, size, fill volume: glass; 30 cm x 22 cm x 24 cm; 10 L
- Aeration: continuous aeration with oil-free air
- No. of organisms per vessel: 5 resp. 10 (5 fishs at 5000 and 10000 mg/L and 10 fishs for 0 and 1000 mg/L)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 4.8 resp. 9.6 g fish/L test water

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstitued fresh water according to DIN 38412 (preparation from fully demineralized tap water)
- Na/K ratio: 10:1
- Alkalinity: 0.8 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 10 µ MHO
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light/8 hours dark

EFFECT PARAMETERS MEASURED: Determination of mortality and symptoms; determination or calculation of the median lethal concentration (LC50) and, if possible, the LC5 and the LC95, using the probit analysis after hours (nominal conc.): 1, 4, 24, 48, 72, and 96 hours.


TEST CONCENTRATIONS
- Justification for concentrations than requested by guideline: In view of the objective, the requirements of the guideline and as no range finding study had been performed the concentrations were fixed at 1000, 5000 and 10000 mg/L. The concentrations exceeded the normal upper limit for acute studies in fish because it was the aim to test higher concentrations for classification of water pollution.

Reference substance (positive control):
yes
Remarks:
chloroacetamide
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: No
- Mortality of control: No
- Abnormal responses: No
Results with reference substance (positive control):
- Mortality: yes
- LC50: after 48 hours 38 mg/L
- Other: The lethal concentration corresponds to the normal sensitivity.

Table 1: Results on effect determination (mortality and symptoms)























































































Nominal Conc. (mg/L)


Number of fish



Dead fish after



1 h



4 h



24 h



48 h



72 h



96 h



1000



5



0



0



0



0



0



0



5000



5



0



0



0



0



0



0



10000



10



0



0



0



0



0



0



0



10



0



0



0



0



0



0



Nominal Conc. (mg/L)


Number of fish



Symptoms



1 h



4 h



24 h



48 h



72 h



96 h



1000



5



No symptoms detectable



5000



5



10000



10



0



10


Validity criteria fulfilled:
not specified
Conclusions:
The test item was tested for acute toxicity to freshwater fish in a 96h-static test. An LC50 of greater than 10000 mg/L and a NOEC of 10000 mg/L have been derived for the 25 % aqueous solution.
Executive summary:

The test item was assessed in a short-term toxicity study for fish in a method that closely followed the guideline of DIN 38 412 ", using a static procedure. Leuciscus idus, Golden orfes were exposed to the test item in a static test for 96 hours at nominal concentrations of 1000, 5000 and 10000 mg/L. No mortality occurred up to the highest tested concentration. No abnormal behaviour was observed. The LC50 of > 10000 mg/L and a NOEC of 10000 mg/L have been derived for the 25 % aqueous solution.

Description of key information

The test item was tested for acute toxicity to freshwater fish in a 96h-static test. An LC50of greater than 10000 mg/L and a NOEC of 10000 mg/L have been derived for the 25 % aqueous solution.

Key value for chemical safety assessment

LC50 for freshwater fish:
10 000 mg/L

Additional information

key study

The test item was assessed in a short-term toxicity study for fish in a method that closely followed the guideline of DIN 38 412 ", using a static procedure. Leuciscus idus, Golden orfes were exposed to the test item in a static test for 96 hours at nominal concentrations of 1000, 5000 and 10000 mg/L. No mortality occurred up to the highest tested concentration. No abnormal behaviour was observed. The LC50 of > 10000 mg/L and a NOEC of 10000 mg/L have been derived for the test solution containing 25 % of the test substance. Based on this study, the derived LC50 and NOEC for 100 % of the test item were > 2500 mg/L and 2500 mg/L, respectively.

 

 

supporting study

Sodium vinylsulphonate was assessed in a short-term toxicity study with fish according to OECD guideline 203. Guppies were exposed to the test item for 24 hours. The EC0 was greater than 500 mg/L based on the lack of mortality and sublethal effects at this concentration and lower for the 30 % test solution.