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Short-term toxicity to fish

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Description of key information

The toxicity of C16-22 alkylamine was tested on Danio Rerio in accordance with OECD 203 in natural river water.

Key value for chemical safety assessment

LC50 for freshwater fish:
3.54 mg/L

Additional information

The acute toxicity of the test item C16-22-(even numbered)-alkylamines (batch no.:S-001453) to fish (zebrafish) was determined according to OECD-Guideline for Testing of Chemicals No. 203 (1992) during June 2013.

A semi-static test with renewal of the test media after 48 h was performed with the nominal concentrations of
0.625 - 1.25 - 2.50 - 5.00 - 10.0 mg/L. Duration of the test was 96 hours. 7 test organisms were exposed to each concentration level and the control. Water quality parameters pH-value, temperature and oxygen-saturation, measured after 0, 24, 48, 72 and 96 h, were determined to be within the acceptable limits.

The test itemC16-22-(even numbered)-alkylamineshas a low water solubility and sorbs to organic and inorganic materials by different mechanisms. The sorption processes are mostly non-linear, means are concentration dependent. Due to these properties the test item is difficult to test in artificial water (e.g. sorption to the test organisms and glass walls of the test vessels) and results deriving from such tests depend on the test settings applied. Using natural river water which contains particulate as well as dissolved organic carbon to which the test item can sorb partially reduces the difficulties encountered in tests with synthetic water (e.g. preventing that the test item settles onto surfaces). The sorbed fraction of the test item was difficult to extract from the test system which normally leads to low analytical recoveries.Due to the short exposure period, these low analytical recoveries cannot be associated to biodegradation. This means that the test substance was for more than 80 % present in the test system and therefore available for exposure (dissolved in water and sorbed also called bulk). This so called Bulk Approach is described by ECETOC (2003). Due to the full exposure of the organisms the test item nominal concentrations were used to base the dose response on instead of measured ones.

All effect levels are given based on nominal concentrations of C16-22-(even numbered)-alkylamines. The test item C16-22-(even numbered)-alkylamines was found to be toxic to zebrafish after 96 h. At the nominal test item concentration of 2.50 mg/L no mortality or non lethal effects were observed. Therefore the NOEC was laid down as 2.50 mg/L. The LC50 after 96 hours is 3.54 (2.50 – 5.00) mg/L.