Potassium iodide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental result performed using standard methods of analysis

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

As no mortalities were found in 96 hrs, the experiment was continued up to seven days and the no observed effect concentration (NOEC) was noted.

Principles of method if other than guideline:

Short term toxicity study of test chemical were carried out on the Danio rerio (previous name: Brachydanio rerio). Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring test water. Deionized water was used

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra Fish
- Age at study initiation: 3-4 months
- Length at study initiation (mean): 2.3 cm
- Weight at study initiation (mean): 0.203 g
- Feeding during test: Yes
- Food type: Standard brand feed
- Frequency: once daily

- Housing: The fishes were kept in a static tank in tap water, under natural conditions along with proper feed and aeration.
- Duration of housing :15 days
- Photoperiod:12 hours light and 12 hours darkness
- Oxygen content:7.4
- pH: Between 6-7
- Water temperature: 29°C
- Mortality (%): 0%

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

As no effects were observed after 96 hrs, test extended for 7 days.

Test temperature:

29°C

pH:

6-7

Dissolved oxygen:

6-7.9 mg/L

Nominal and measured concentrations:

100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass bowl aquaria
- Material, size, headspace, fill volume: 12” glass bowl aquaria were used
- Aeration: Aeration was provided 1 hr before the starting of experiment and also during the experiment when requiredWithdrawal of food: 1 day before and during exposure
- No. of organisms per vessel: 8 fishes

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No effects were observed

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Other details not known

Duration:

7 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Other details not known

Sublethal observations / clinical signs:

Mortality and symptoms

Nominal conc. (mg/l)	Number of fish	Mortality (cumulated) after
		1^stDay	2^ndDay	3^rdDay	4^thDay	5^th Day	6^thDay	7^thDay
0 (control)	8	0	0	0	0	0	0	0
100	8	0	0	0	0	0	0	0

Nominal conc. (mg/l)	Number of fish	Symptoms after
		1^stDay	2^ndDay	3^rdDay	4^thDay	5^th Day	6^thDay	7^thDay
0 (control)	8	NS8	NS8	NS8	NS8	NS8	NS8	NS8
100	8	NS8	NS8	NS8	NS8	NS8	NS8	NS8

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

Validity criteria fulfilled:

yes

Conclusions:

Based on the mortality of Danio rerio (previous name: Brachydanio rerio) by the test chemical after 96 hrs of exposure, LC50 was observed to be > 100 mg/l. As no effect were observed at 100 mg/l test extended for 7 days. After 7 days of exposure no effect were observed.

Executive summary:

Short term toxicity study of test chemical were carried out on the Danio rerio (previous name: Brachydanio rerio). Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The limit test at 100 mg/L was conducted. The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring test water. Deionized water was used. Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. The test vessels used were all glass bowl aquaria of 12” each. 3-4 months old 8 fishes in each vessel were added. In a 96 h study for test chemical on Danio rerio, at a test concentration of 100 mg/L, the fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l. No mortalities were observed in the control aquaria. Based on the mortality of Danio rerio (previous name: Brachydanio rerio) by the test chemical after 96 hrs of exposure, LC50 was observed to be > 100 mg/l. As no effect were observed at 100 mg/l test extended for 7 days. After 7 days of exposure no effect were observed. Thus on the basis of LC50 > 100 mg/l, chemical consider to be nontoxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Short term toxicity study of test chemical were carried out on the Danio rerio (previous name: Brachydanio rerio). Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The limit test at 100 mg/L was conducted. The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring test water. Deionized water was used. Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. The test vessels used were all glass bowl aquaria of 12” each. 3-4 months old 8 fishes in each vessel were added. In a 96 h study for test chemical onDanio rerio, at a test concentration of 100 mg/L, the fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l. No mortalities were observed in the control aquaria. Based on the mortality of Danio rerio (previous name: Brachydanio rerio) by the test chemical after 96 hrs of exposure, LC50 was observed to be > 100 mg/l. As no effect were observed at 100 mg/l test extended for 7 days. After 7 days of exposure no effect were observed. Thus on the basis of LC50 > 100 mg/l, chemical consider to be nontoxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Data available for the test chemicals including structurally and functionally similar read across chemicals, study has been reviewed to determine the short term toxicity on fish of the test chemical. The studies are as mentioned below:

 

In the first study short term toxicity of test chemical were carried out on the Danio rerio (previous name: Brachydanio rerio). Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The limit test at 100 mg/L was conducted. The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring test water. Deionized water was used. Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. The test vessels used were all glass bowl aquaria of 12” each. 3-4 months old 8 fishes in each vessel were added. In a 96 h study for test chemical on Danio rerio, at a test concentration of 100 mg/L, the fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l. No mortalities were observed in the control aquaria. Based on the mortality of Danio rerio (previous name: Brachydanio rerio) by the test chemical after 96 hrs of exposure, LC50 was observed to be > 100 mg/l. As no effect were observed at 100 mg/l test extended for 7 days. After 7 days of exposure no effect were observed. Thus on the basis of LC50 > 100 mg/l, chemical consider to be nontoxic and can be consider to be not classified as per the CLP classification criteria.

 

Above study was supported by the second study. Short term toxicity of test chemical were determine on the growth of fishes Oncorhynchus mykiss (previous name: Salmo gairdneri). Test conducted under the static system for 96 hrs. The lethal concentration (LC50) value of test chemical on fish (Oncorhynchus mykiss) in a 96 hrs study on mortality effect was determine to be 2190 mg/L. Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Oncorhynchus mykiss).

 

In the third study from secondary source short term toxicity of test chemical were determine on the growth of fish Oncorhynchus mykiss (previous name: Salmo gairdneri). Test conducted under the static system on 50 MM Oncorhynchus mykiss. 96 hrs of incubation were provided to the fishes. After the exposure of test chemical with Oncorhynchus mykiss (previous name: Salmo gairdneri) for 96 hrs, LC50 was observed to be 896 mg/l. Based on the LC50 value, chemical consider to be nontoxic and not classified as per the CLP classification criteria.

 

Based on the above studies, chemical concluded to be nontoxic and thus can be consider to be not classified as per the CLP classification criteria.