Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 222-321-7 | CAS number: 3425-61-4
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
There is no test performed with Tert-amyl hydroperoxide. The toxic effects on microorganisms of this substance is read-across from data obtained with1,1,3,3-Tetramethylbutyl hydroperoxide (OECD 209 guideline): EC50-3h = 138 mg/L, EC10-3h = 33 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 138 mg/L
- EC10 or NOEC for microorganisms:
- 33 mg/L
Additional information
There is no test performed with Tert-amyl hydroperoxide. The toxic effects on microorganisms of this substance is read-across from data obtained with1,1,3,3-Tetramethylbutyl hydroperoxide.
In order to predict effects of chemicals in the environment and in biological waste water treatment plants, an activated sludge respiration inhibition test was performed. The toxicity to activated sludge was determined in accordance with OECD Test Guideline 209, and in compliance with the OECD principles of Good Laboratory Practice. The reference substance and test substance were measured on two subsequent days. The test is valid as shown by the EC50 of the reference compound, 3,5- dichlorophenol (8 mg/L), the coefficient of variation of the replicates of the control oxygen uptake rates (2% on first day and 3% on second day) and the average oxygen uptake rate for the control replicates of 15 mg O2/g dry weight/hour on the first day and 26 mg O2/g dry weight/hour on the second day. The oxygen uptake rate on the first day is slightly lower than prescribed. Accurate oxygen uptake rate measurements were very well possible as shown by the very low coefficient of variation. Therefore this is a minor deviation which does not invalidate the test. The toxicity of 1,1,3,3-tetramethylbutyl hydroperoxide to activated sludge was determined at a contact time of 3 hours, using various concentrations of the test substance. The inhibitory effect of 1,1,3,3-tetramethylbutyl hydroperoxide at a particular concentration is expressed as a percentage of the two controls.
The EC50 of 1,1,3,3-tetramethylbutyl hydroperoxide for activated sludge after 3 hours contact time is 138 mg/L with 95% confidence limits of 53 and 327 mg/L. The EC10 of 1,1,3,3-tetramethylbutyl hydroperoxide for activated sludge after 3 hours contact time is 33 mg/L with 95% confidence limits of 4 and 78 mg/L. The EC20 and EC80 are 54 and 349 mg/L, respectively.1,1,3,3-tetramethylbutyl hydroperoxide is therefore considered harmless to activated sludge.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
