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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The test substance is poorly soluble and made of constituents with various water solubility. As a consequence, the exposures were performed with Water Accommodated Fractions (WAFs). Therefore, the results are based on nominal loadings and the LC50 value given below is actually a LL50 (lethal loading) value. No LC50 could be derived from the acute toxicity studies.
The 48h-LL50 is > 100 mg/l.

Key value for chemical safety assessment

Additional information

Twelve study reports were available and input as endpoint summary records, including a recent Daphnia magna study with the registration substance itself.

Measured acute (short-term) toxicity data are available for Shell GTL Solvent GS270 (Hydrocarbons, C15-C19, n-alkanes, isoalkanes, <2% aromatics) to the freshwater invertebrate Daphnia magna (Harris, 2014). The test was conducted under static (no renewal of the test media) conditions in accordance with OECD Test Guideline 202 and method C2 of EC Regulation No. EC 440/2008. Appropriate modifications to the test and media preparation procedures were made to take account of the test substance containing multiple constituents, having low solubility in water and being potentially volatile. No effects on mobility of D. magna were observed after 48 hours exposure to the test medium prepared as a water-accommodated fraction (WAF) at loading rates up to 100 mg/l; 48-hour EL50>100 mg/l, NOELR ≥100 mg/l. Samples of the control and the 10 and 100 mg/l loading rate WAFs were taken at 0 and 48 hours for Total Organic Carbon (TOC) analysis. Given the background level of carbon in the control vessels and also the low level of carbon in the test vessels, it was considered that all the results were around the limit of quantification of the analytical method. The results of the test are considered to be reliable.

All of the other studies investigate the toxicity of the substance “Hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, = 2% aromatics” on a marine copepod, Acartia tonsa exposed to water accommodated fractions (WAFs). Several studies indicate very high values (up to 69 155 mg/l in Altin, 1998b), In most of the studies the calculation of LL50 could not be achieved

because of the low mortality levels obtained (Aunaas, 1996a and c; Larsen, 2006a, b, d and e; 2008a-c).