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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Remarks:
The tested concentration (0.26 mg/L) is low.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report Date:
1980

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Principles of method if other than guideline:
BASF test (Inhalation hazard test). The test was performed in priniple as described in OECD Guideline 403. The test demonstrates the toxicity of an atmosphere saturated with vapours of the volatile components of a test substance at the temerature chosen for vapour generation (20°C). 3 rats per sex were exposed sequentially to the vapours, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted gassdisc in a glass cylinder for 8h. The documentation of clinical signs was performed over a period of 7 days. In order toverify the results, the test was repeated once.
GLP compliance:
no
Test type:
other: Inhalation-risk test (IRT)
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2-Methylimidazol in Schuppen
- Substance number: 77/697

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Firma Wiga, Sulzfeld
- Diet: Herilan MRH (firma: EGGERSMANN, KG, Rinteln), ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
not specified
Vehicle:
air
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Temperature in air chamber: 20°C
- Vapour generation: generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted gassdisc in a glass cylinder for 8 hours.
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
7 h
Concentrations:
0.26 mg/L
No. of animals per sex per dose:
6
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs and body weight

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC0
Effect level:
> 0.26 mg/L air
Based on:
test mat.
Remarks:
(initial - final substance weight) / (200 l/h * 7h)
Exp. duration:
7 h
Remarks on result:
other: none of the animals died
Mortality:
None of the animals died during the observation period.
Clinical signs:
other: No abnormalities were detected.
Body weight:
Normal body weight gain was observed.
Gross pathology:
No abnormalities were detected.

Applicant's summary and conclusion