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Short-term toxicity to aquatic invertebrates: Time [h]  EC 0 [mg/l]    24  ≥ 100 mg/I    48  ≥ 61.6 mg/I


The results are expressed in terms of nominal concentrations (at 24h), and in terms of mean measured concentrations (at 48h). Measured concentrations correspond to 73.6% of nominal values at 0 hours, and to 49.6% of nominal values at 48 hours, respectively.

Short-term toxicity to fish: The acute fish toxicity (96h LC50) of Ethyl 2-methylbutyrate Common carp, (Cyprinus carpio) was found to be greater than 100 mg a.i./L.

Toxicity to aquatic algae and cyanobacteria:As the study was conducted as a limit test the EC50, growth inhibition, growth reduction, NOEC and LOEC of Ethyl 2-methylbutyrate could not be calculated. The Ethyl 2-methylbutyrate does not inhibit the growth of alga (Pseudokirchneriella subcapitata) up to the 100 mg/L of alga media.

Long-term toxicity to aquatic invertebrates:The No Observed Effective Concentration (NOEC) of Ethyl 2-methylbutyrate tested at which no statistically significant difference in reproductive output was observed was 1.3 mg/L, as compared with vehicle control. The Lowest Observed Effective Concentration (LOEC) tested at which statistically significant difference in reproduction output was observed was 3.6 mg/L, as compared with vehicle control. The total numbers of offspring per group were recorded and analysed statistically. There was a significant decrease (p ≤ 0.01) in the production of offspring during exposure period at all of the test concentrations except the lowest (1.3 mg Ethyl 2-methylbutyrate/L) as compared with the vehicle control group. The 21 day EC50 for reproductive output in Daphnia magna with Ethyl 2-methylbutyrate was 22.53 mg/L with the 95% fiducial limits between 5.48 and 92.67 mg/L. The regression equation established [Probit mortality (y) vs log concentration (mg/L) of Ethyl 2-methylbutyrate (x)] was y = 0.99 + 0.92x.

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