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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short-term toxicity to aquatic invertebrates: Time [h]  EC 0 [mg/l]    24  ≥ 100 mg/I    48  ≥ 61.6 mg/I


The results are expressed in terms of nominal concentrations (at 24h), and in terms of mean measured concentrations (at 48h). Measured concentrations correspond to 73.6% of nominal values at 0 hours, and to 49.6% of nominal values at 48 hours, respectively.

Short-term toxicity to fish: The acute fish toxicity (96h LC50) of Ethyl 2-methylbutyrate Common carp, (Cyprinus carpio) was found to be greater than 100 mg a.i./L.

Toxicity to aquatic algae and cyanobacteria:As the study was conducted as a limit test the EC50, growth inhibition, growth reduction, NOEC and LOEC of Ethyl 2-methylbutyrate could not be calculated. The Ethyl 2-methylbutyrate does not inhibit the growth of alga (Pseudokirchneriella subcapitata) up to the 100 mg/L of alga media.

Long-term toxicity to aquatic invertebrates:The No Observed Effective Concentration (NOEC) of Ethyl 2-methylbutyrate tested at which no statistically significant difference in reproductive output was observed was 1.3 mg/L, as compared with vehicle control. The Lowest Observed Effective Concentration (LOEC) tested at which statistically significant difference in reproduction output was observed was 3.6 mg/L, as compared with vehicle control. The total numbers of offspring per group were recorded and analysed statistically. There was a significant decrease (p ≤ 0.01) in the production of offspring during exposure period at all of the test concentrations except the lowest (1.3 mg Ethyl 2-methylbutyrate/L) as compared with the vehicle control group. The 21 day EC50 for reproductive output in Daphnia magna with Ethyl 2-methylbutyrate was 22.53 mg/L with the 95% fiducial limits between 5.48 and 92.67 mg/L. The regression equation established [Probit mortality (y) vs log concentration (mg/L) of Ethyl 2-methylbutyrate (x)] was y = 0.99 + 0.92x.

Additional information