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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

96 h LC50: > 10 mg/L

Key value for chemical safety assessment

LC50 for freshwater fish:
10 mg/L

Additional information

In the Klimisch 1 GLP study from Kimmel (2013) theacute toxicity of Aspartic acid, N-(3-carboxy-1-oxo-sulfopropyl)-N-(C16-C18 (even numbered), C18 unsaturated alkyl) tetrasodium salts to zebra fish (Danio rerio) was determined in a 96‑hour semi-static test with daily test medium renewal according to the EU Commission Directive 92/69/, Part C.1, the Commission Regulation (EC) No 440/2008, Part C.1 and the OECD Guideline for Testing of Chemicals No. 203 (1992). The nominal test item concentrations tested were 0.29, 0.93, 2.92, 9.33 and 29.17 mg test item/L. corresponding to 0.1, 0.32, 1.0, 3.2 and 10 mg solid content/L (mg solid content/L). Additionally, a control group was tested in parallel. In the analyzed test medium samples from the start and end of the first and last test medium renewal periods the measured test item concentrations ranged between 97 and 100% of the nominal value. Consequently, the test water renewal ensured nearly constant concentration of the test item during the test and the reported biological results are related to the nominal test item concentration.In the control and up to and including the highest test concentration 10 mg solid content/L, all fish survived until the end of the test and no visible abnormalities were observed.The 96 hour NOEC and LC0of the test item to zebra fish were both determined to be 10 mg solid content/L. The 96 hour LC50of the test item was determined to be > 10 mg solid content/L.

The results of this study are considered relevant and reliable for the risk assessment.