Tris(oxiranylmethyl) benzene-1,2,4-tricarboxylate

Endpoint summary

Additional information

Tris(oxiranylmethyl)benzene-1,2,4-tricarboxylate is mutagenic in vitro, which is not surprising considering the three epoxy groups and knowing that most of the epoxy compounds are known to be mutagenic under in vitro conditions. However, negative results were obtained in two in vivo studies; an in vivo micronucleus study performed with rats and an in vivo germ-cell cytogenetics study performed with mice. Tris(oxiranylmethyl)benzene-1,2,4-tricarboxylate is therefore considered as negative for mutagenicity.

Short description of key information:
tris(oxiranylmethyl)benzene-1,2,4-tricarboxylate was found to be mutagenic in vitro in a bacterial reverse mutation assay performed according to OECD 471 as well as in mammalian cell gene mutation assay at the Thymidine kinase (tk)-Locus performed according to OECD 476.
However, tris(oxiranylmethyl)benzene-1,2,4-tricarboxylate was found to be not clastogenic in vivo in a micronucleus assay in rat bone marrow cells performed according to OECD 474 and in a mammalian germ-cell cytogenetics assay in mice performed according to OECD 483.

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

Based on the above stated assessment of the genotoxic potential tris(oxyranylmethyl)benzene-1,2,4-tricarboxylate (Ames test positive,in vitro mammalian cell gene mutation test positive, in vivo mammalian micronucleus test in bone marrow cells negative and in vivo spermatogonia chromosome aberration test negative) the substance is deemed non-genotoxic and accordingly does not need to be classified according to Council Directive 2001/59/EC (28th ATP of Directive 67/548/EEC) and according to CLP (Regulation (EC) No 1272/2008 Of The European Parliament And Of The Council) as implementation of UN-GHS in the EU.