Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988/09/26 to 1988/09/30
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP study, without analytics.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
Non-GLP study, without analytics.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance and dispersant were added directly into the tank and homogenously distributed. The fish were then added to the tank.
- Controls: Blank - water; dispersant - 0.67 mg/L alkylphenol polyglycol ether
- Chemical name of dispersant: alkylphenol polyglycol ether
- Concentration of dispersant in test medium (stock solution and final test solution(s) including control(s)): 0.64 mg/L added directly to the water in the tank
- Evidence of undissolved material (e.g. precipitate, surface film, etc): precipitate noted after 24 h
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium/D-3422 Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): 19-27 mm
- Weight at study initiation (mean and range, SD): 0.05-0.17 g
- Method of breeding: no data
- Feeding during test: no

ACCLIMATION
- Acclimation period: 19 days
- Acclimation conditions (same as test or not):
- Type and amount of food: no data
- Feeding frequency: no food 24 hours prior to exposure
- Health during acclimation (any mortality observed): no data

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
180 mg CaCO3/L
Test temperature:
23+/-1
pH:
8.1-8.3
Dissolved oxygen:
62-103 %
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Type: open
- Material, size, headspace, fill volume: glass, 20L, fill volume = 15 L
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.08 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 hours/8 hours light/dark
- Light intensity: No data, fluorescent light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, visible abnormalities, water quality characteristics, were measured daily
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Behavioural abnormalities: Swimming behaviour in 2/20 at 100 mg/L
- Observations on body length and weight: no data
- Other biological observations: none
- Mortality of control: none in blank or dispersant controls
- Other adverse effects control: none in blank or dispersant controls
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Precipitation observed after 24 hours
- Effect concentrations exceeding solubility of substance in test medium: yes

Description of key information

With high probability acutely not harmful to fish. No toxic effects in the range of water solubility. 

Key value for chemical safety assessment

Additional information

In a 96-h acute toxicity study, zebra-fish (Brachydanio rerio) were exposed to test item at nominal concentrations of 0 (control, solvent control) and 100 mg/L under static conditions. The 96-h LC50 was > 100 mg/L. The NOEC, based on mortality and sublethal effects, were ≥ 100 mg/L, respectively. Sublethal effects were not observed. It can be concluded that the test substance is not acutely toxic to fish in the range of solubility.