Anthraquinone

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Although the study was not conducted according to the recommended guidelines, it provides scientific valid information to assess the metabolism of the substance.

Data source

Reference

Reference Type:

publication

Title:

Metabolism of Polycyclic Compounds. The metabolism of anthracene and some related compounds in rats

Author:

Sims P

Year:

1964

Bibliographic source:

Biochem. J. 92, 621-631

Materials and methods

Objective of study:

metabolism

Principles of method if other than guideline:

Four rats were maintained for 4 days on a diet containing 5% (w/w) of the test substance, the urines being collected daily.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Anthraquinone

EC Number:

201-549-0

EC Name:

Anthraquinone

Cas Number:

84-65-1

Molecular formula:

C14H8O2

IUPAC Name:

9,10-dihydroanthracene-9,10-dione

Radiolabelling:

no

Test animals

Species:

rat

Strain:

other: Chester-Beatty

Sex:

male

Administration / exposure

Route of administration:

oral: feed

Duration and frequency of treatment / exposure:

4 days

Doses / concentrations

Remarks:

Doses / Concentrations:
5% w/w

No. of animals per sex per dose / concentration:

4 males

Control animals:

no

Results and discussion

Metabolite characterisation studies

Metabolites identified:

yes

Details on metabolites:

Sulfate and glucuronide conjugates of 2-hydroxyanthraquinone, 9,10-dihydroxyanthracene, and 2,9,10-trihydroxyanthracene were found in pooled urine samples collected over the 4 days.

Applicant's summary and conclusion

Conclusions:

Sulfate and glucuronide conjugates of 2-hydroxyanthraquinone, 9,10-dihydroxyanthracene, and 2,9,10-trihydroxyanthracene were found in pooled urine samples collected over the 4 days.

Executive summary:

Four rats were maintained for 4 days on a diet containing 5% (w/w) of the test substance, the urines being collected daily. The combined urines were acidified with acetic acid and treated with charcoal (25 g). The charcoal was filtered off and washed with water (500 ml) and the absorbed material was eluted with 1 l of methanol-aq. NH3 (19:1, v/v). The solvent was evaporated and the residue chromatographed on six sheets of Whatman no. 3MM chromatography paper for 18 h with butan-1-ol-propan-1-ol-2N-NH3 (2:1:1, by vol.). A number of fluorescent bands were seen when the chromatograms were examined in UV light, and these were cut out and the absorbed materials were eluted from the paper with methanol-aq. NH3 (19: 1, v/v). Removal of the solvent gave a number of fractions as gums that were examined on paper chromatograms. The products obtained after chemical and enzymic hydrolyses of the fractions were examined on thin-layer chromatograms. Sulfate and glucuronide conjugates of 2-hydroxyanthraquinone, 9,10-dihydroxyanthracene, and 2,9,10-trihydroxyanthracene were found in pooled urine samples collected over the 4 days.