Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003-01-16 to 2003-03-17
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study with analytical monitoring

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0, 12.5, 25, 50 and 100 mg/L nominal
- Sampling method: At the start of the test (after 0 h, without daphnids) and at the end of the test (after 48 h) samples from vessels with daphnids were taken.


Test solutions

Vehicle:
no
Details on test solutions:
- Preparation of the stock solution and dilution: The test substance was stirred in M4 medium for about 20 hours at 20 ± 2°C. The concentration of the stock solution was 200,1 mg/2L. By diluting this stock solution with M4 medium the desired nominal concentrations were prepared.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Source: National de Recherche Chimique Appliquée, France
- Age at study initiation: 2 - 24 h (starting with the 3rd breed of parent animals)
- Age of stock animals: 2 - 4 weeks
- Feeding during test: None

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.20 - 3.20 mmol/L
Test temperature:
19.6 - 20.3 °C
pH:
8.0 - 8.2
Dissolved oxygen:
8.7 - 9.1 mg/L
Nominal and measured concentrations:
Nominal: 12.5, 25, 50 and 100 mg/L
Measured concentrations were within ± 5 % of the nominal values. Therefore, the results were based on nominal concentrations.
Details on test conditions:
TEST SYSTEM
- Test vessel: Covered beakers with a nominal volume of 100 mL
- Fill volume: 50 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to guideline
- Intervals of water quality measurement: At test start and at test end

OTHER TEST CONDITIONS
- Photoperiod: day:night rhythm = 16 : 8 h
- Light intensity: About 1 to 8 µE/(m2*s) at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED: Mobility

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: None

Any other information on results incl. tables

Measured concentrations confirm the nominal values. Therefore, the following results were based on nominal concentrations.

No sublethal effects could be observed. No other effects were observed.

Applicant's summary and conclusion