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Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from study report

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
Principles of method if other than guideline:
Acute oral toxicity of test chemical in mice.
GLP compliance:
not specified
Test type:
other: not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2,2-trichloro-1-phenylethyl acetate
EC Number:
201-972-0
EC Name:
2,2,2-trichloro-1-phenylethyl acetate
Cas Number:
90-17-5
Molecular formula:
C10H9Cl3O2
IUPAC Name:
2,2,2-trichloro-1-phenylethyl acetate
Test material form:
solid: particulate/powder
Details on test material:
Test material identity: 2,2,2-trichloro-1-phenylethyl acetate/ 90-17-5/ 201-972-0
- IUPAC Name: 2,2,2-trichloro-1-phenylethyl acetate
- Common Name: Rosacetone
- InChI: 1S/C10H9Cl3O2/c1-7(14)15-9(10(11,12)13)8-5-3-2-4-6-8/h2-6,9H,1H3
- Smiles: c1([C@@H](OC(C)=O)C(Cl)(Cl)Cl)ccccc1
Details on test material
- Name of test material:Rosacetone
- Molecular formula :C10H9Cl3O2
- Molecular weight :267.538 g/mol
- Substance type:Organic
- Physical state:Solid – Fine white crystalline powder

Test animals

Species:
mouse
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Age at study initiation: 4-5 week old
- Fasting period before study: 4 hours
- Housing: The animals were housed individually in cages.
- Diet (e.g. ad libitum): pelleted diet, ad libitum
- Water (e.g. ad libitum): water, ad libitum

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: groundnut oil
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 20% w/v solution
Doses:
2000, 5000 and 10000 mg/kg
No. of animals per sex per dose:
Total = 10
2000 mg/kg - one male and one female
5000 mg/kg - three male and three female
10000 mg/kg - one male and one female
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: The animals were observed for signs of toxicity for 7 days. Survivors were weighed before killing for post-mortem examination at the end of the one week observation period.
- Necropsy of survivors performed: yes, all animals dying were autopsied. All survivors were killed and examined post mortem after 7 days.
Statistics:
The approximate LD50 value is estimated from the results and the test substance is given a toxicity rating according to Hodge and Sterner's classification.

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 - < 5 000 mg/kg bw
Based on:
test mat.
Remarks on result:
other: no further details available
Mortality:
At 2000 mg/kg – No mortality was observed.
At 5000 mg/kg – 1 mouse died within 24 hours and 1 mouse died within 96 hours after treatment.
At 10000 mg/kg – Both the mice were died within 48 and 66 hours.
Clinical signs:
other: At 2000 mg/kg – The male mouse was somnolent, showing signs of stress and exhibiting labored breathing within 18 hours after treatment but recovered within 42 hours. The female mouse appeared unaffected by the treatment. At 5000 mg/kg – The mice were show
Gross pathology:
Autopsy of the animals that died revealed gaseous distension and irritation of the stomach and intestines. The mice dying after 48 hours and later had pale soft rose-pink livers, pale spleens and mottled kidneys. Hemorrhaging was also present in the ileum of these mice.
Other findings:
No data

Applicant's summary and conclusion

Interpretation of results:
other: Not classified
Conclusions:
The acute oral toxicity dose LD50 value was considered in between 5000-10000 mg/kg bw, when 10 male and female White mice were treated with test chemical via oral gavage route.
Executive summary:

The acute oral toxicity of test chemical was tested in 10 male and female White mice at thedose concentration of 2000, 5000 and 10000 mg/kgbw. The given test chemical was dissolved as 20% w/v solutionin groundnut oil and administered via oral gavage route. The animals were observed for signs of toxicity for 7 days. Survivors were weighed before killing for post-mortem examination at the end of the one week observation period. All animals dying were autopsied. All survivors were killed and examined post mortem after 7 days.The approximate LD50 value is estimated from the results and the test substance is given a toxicity rating according to Hodge and Sterner's classification. Mortality was observed as - At 2000 mg/kg – No mortality was observed. At 5000 mg/kg – 1 mouse died within 24 hours and 1 mouse died within 96 hours after treatment. At 10000 mg/kg – Both the mice were died within 48 and 66 hours. Clinical signs were observed such as - At 2000 mg/kg – The male mouse was somnolent, showing signs of stress and exhibiting labored breathing within 18 hours after treatment but recovered within 42 hours. The female mouse appeared unaffected by the treatment. At 5000 mg/kg – The mice were showing signs of stress and exhibiting lacrimation within 30 mins after treatment and within 2 hours somnolence and laboured breathing was also observed. These mice were comatose within 18-42 hours after treatment. The surviving mice continued to show signs of stress throughout the 7 day observation period. At 10000 mg/kg – The mice were showing signs of stress and exhibiting lacrimation within 30 mins after treatment and within 2 hours somnolence and laboured breathing was also observed. These mice were comatose within 18-42 hours after treatment. Surviving animals gained weight during the 7 day observation period and presented a normal appearance at autopsy. Autopsy of the animals that died revealed gaseous distension and irritation of the stomach and intestines. The mice dying after 48 hours and later had pale soft rose-pink livers, pale spleens and mottled kidneys. Hemorrhaging was also present in the ileum of these mice. Therefore, LD50 value was considered in between 5000-10000 mg/kg bw, when 10 male and female White mice were treated with test chemical via oral gavage route.