N-acetylsulphanilyl chloride

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

experimental data of read across substances

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on four toxicity to microorganism studies as-
WoE 2, WoE 3 and WoE 4.

GLP compliance:

not specified

Analytical monitoring:

not specified

Remarks:

WoE 2: not specified, WoE 3: no and WoE 4: not specified

Vehicle:

not specified

Test organisms (species):

other: WoE 2 and WoE 3: activated sludge of a predominantly domestic sewage and WoE 4: anaerobic bacteria

Test type:

not specified

Water media type:

not specified

Total exposure duration:

3 h

Remarks on exposure duration:

WoE 2 and WoE 3: 3 hr, WoE 4: 24 hr

Reference substance (positive control):

not specified

Duration:

3 h

Dose descriptor:

EC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks on result:

other: WoE 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks on result:

other: WoE 2

Duration:

3 h

Dose descriptor:

EC0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks on result:

other: WoE 3

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

> 5 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: WoE 4

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of the various experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 3 hr EC50 value can be expected to be > 500 mg/l.

Executive summary:

Data available of the structurally and functionally similar read across chemicals has been reviewed to determine the effect of the test chemical on toxicity to microorganisms or activated sludge. The studies are as mentioned below:

Toxicity study to activated sludge was carried out for 3 hrs for assessing the effect of test chemical. The study was performed in accordance with the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test. Activated sludge, predominantly domestic sewage was used as a test organism. On the basis of the effect on respiration rate of the test inoculum, the 3 hr EC0 and EC50 value was determined to be 500 and >500 mg/l, respectively.

Another toxicity study to activated sludge was carried out for 3 hrs for assessing the effect of test chemical. The study was performed following the principles of the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test. Activated sludge, predominantly domestic sewage was usedas a test organism. Test chemical concentrations were not analytically verified. On the basis of the effect on respiration rate of the test inoculum, the 3 hr EC0 value was determined to be >1000 mg/l (nominal concentrations).

For the test chemical, toxicity study to anaerobic bacteria was carried out for 24 hrs for assessing the effect of test chemical. On the basis of the effect on growth inhibition of the test bacteria, the 24 hr EC10 value was determined to be >5000 mg/l.

On the basis of the various experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 3 hr EC50 value can be expected to be > 500 mg/l.

Description of key information

On the basis of the various experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 3 hr EC50 value can be expected to be > 500 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

500 mg/L

Additional information

Data available of the structurally and functionally similar read across chemicals has been reviewed to determine the effect of the test chemical on toxicity to microorganisms or activated sludge. The studies are as mentioned below:

 

Toxicity study to activated sludge was carried out for 3 hrs for assessing the effect of test chemical. The study was performed in accordance with the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test. Activated sludge, predominantly domestic sewage was used as a test organism. On the basis of the effect on respiration rate of the test inoculum, the 3 hr EC0 and EC50 value was determined to be 500 and >500 mg/l, respectively.

 

Another toxicity study to activated sludge was carried out for 3 hrs for assessing the effect of test chemical. The study was performed following the principles of the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test. Activated sludge, predominantly domestic sewage was usedas a test organism. Test chemical concentrations were not analytically verified. On the basis of the effect on respiration rate of the test inoculum, the 3 hr EC0 value was determined to be >1000 mg/l (nominal concentrations).

 

For the test chemical, toxicity study to anaerobic bacteria was carried out for 24 hrs for assessing the effect of test chemical. On the basis of the effect on growth inhibition of the test bacteria, the 24 hr EC10 value was determined to be >5000 mg/l.

 

On the basis of the various experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 3 hr EC50 value can be expected to be > 500 mg/l.