Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jun - Jul 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to OECD guideline under GLP
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
Deviations:
no
Principles of method if other than guideline:
not relevant
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Triamin-Dihydrochlorid
- Analytical purity: 98.4%
- Lot/batch No.: 450790

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
20 mg/L
Based on:
DOC

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
28 d
Details on results:
The reference compound sodium acetate was degraded to 95% on day 5 (~ 4 days of incubation). In the toxicity control, the reference compound
sodium acetate plus the test compound ZK 156454 was degraded to 49% on day 5 (~ 4 days of incubation).

Any other information on results incl. tables

Table 1: Cumulative biological degradation in percent (corrected tor blank values)

Test compound  Day of sampling
1 2 5 8 15 22 28 29
ZK 156454  0 0 1 0 0 0 0 0
Sodium acetate (reference)  0 0 95 89 95 97 97 100
ZK 156454 + sodium acetate  0 0 49 45 45 172 48 47

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
In accordance with the DECD guideline, ZK 156454 is not ready biodegradable.
Additionally, ZK 156454 has no inhibiting effects on the microbial activity since the degradation in the toxicity control was proportional to the
content of the reference compound.
Executive summary:

The purpose of this study was to determine the ready biodegradability of the test compound Triamin-Dihydrochlorid (ZK 156454).

The study was conducted in agreement with the test guideline of the OECD, no. 301 E.

The test substance was incubated in aqueous solutions including nutrients with microorganisms fram a municipal sewage treatment plant for 28 days. The nutrient solution contained phosphates, ammonium chloride, vitamins and trace elements and was added to the test solution. The test substance with a concentration of 20 mg DOC/L (DOC: dissolved organic carbon) was tested in triplicate.

Additionally, a reference substance (sodium acetate) was tested individually at the same DOC concentration and according to the same procedure in order to verify the viability and activity of the degrading micraorganisms. Furthermore, one flask containing the test substance and the reference substance, in each case 20 mg/L as DOC, total 40 mg/L as DOC, was tested as a

toxicity control. Three additional vessels without any test or reference substance were used as blank (control).

The biological degradation of the test and reference substances was evaluated by the decrease of DOC in the solutions. DOC was analysed on days 1, 2, 5, 8, 15, 22, 28 and 29.

The test compound Triamin-Dihydrochlorid showed practically no degradation. The reference compound sodium acetate was degraded to 95% on day 5 (~ 4 days of incubation). In the toxicity control, the reference compound sodium acetate plus the test compound was degraded to 49% on day 5 (~ 4 days of incubation).