Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
For the preparation of a stock solution, 1 g/ I of the test item was equilibrated in synthetic fresh water without NaHC03, stirred about 18 hand finally
filtered off. The filtrate was used as stock solution.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna (Strauss)
- Method of breeding: For breeding, the females are maintained in M4 medium in 1 L beakers. A reddish dark-brown coloration of the animals
indicates that they are healthy. At two to three day intervals, the exuviae are syphoned off and the water is changed. In the course of this, the
offspring is removed from the breeding vessels. At regular intervals of approximately 4 weeks, offspring is isolated from the vessels. They serve as a basis for further breeding. The culture conditions ensure parthenogenetic reproduction.
- Feeding during test: The daily amount of food is such that the amount added is consumed by the animals.
- Food type: algae (Desmodesmus subspicatus)
- Amount: not specified (daphnids were fed with an appropriate amount of algae which could be roughly consumed per day)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
14°dH
Test temperature:
20.5-20.9°C (mean: 20.8°C)
pH:
7.8-7.9
Dissolved oxygen:
7.6-8.4 mg/L
Nominal and measured concentrations:
nominal: 1.4, 2.5, 4.3, 7.5, 13, 22, 39 and 61 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Round-bottom test tubes calibrated to 10 mL
- Aeration: no
- No. of organisms per vessel: 20 organisms in 4 parallel sets of 5 each
- No. of vessels per concentration (replicates): 8 test item concentrations
- No. of vessels per control (replicates): 1 control

TEST MEDIUM / WATER PARAMETERS
compoundl parameter concentration (mgl L)
CaCI2 x 2 H20 294
MgS04 x 6 H20 114
NaHC03 65
KCI 6
sum of Ca2+ - and Mg2+ -ions 2.5 mmol
proportion of Ca2+ - : Mg2+ -ions (mol) 4:1
proportion of Na+- : K+-ions (mol) 10:1
water hardness 14°dH = 250 mg CaC03/ L

OTHER TEST CONDITIONS
Light/dark cycle: dark
Temperature: 20.5 - 20.9°C (mean: 20.8°G)
Aeration: none
Feeding: none
Solubilizer: none
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
7.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
52 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 61 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The analytical verification of the test item concentrations was carried out after 0 and 48 hours.
The measured test concentrations after 48 h did not deviate by more than 20% from the freshly prepared test solutions. It can be concluded that
the test item was present during the whole exposure time. Therefore, the calculated values of the test item concentrations were used for the
evaluation of the test.
Results with reference substance (positive control):
Influence of potassium dichromate on the swimming behaviour after 24 hexposure:
1.0 mg/L 45% of immobilised Daphnia
2.0 mg/L 100% of immobilised Daphnia

Applicant's summary and conclusion

Validity criteria fulfilled:
yes