Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Qualifier:
according to
Guideline:
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Suspended matter: 26.7 mg/L
- Dry matter: 4012 mg/L
- Colony forming units (CFU): 187 * 10^4 mL
Duration of test (contact time):
29 d
Initial test substance concentrationopen allclose all
Initial conc.:
15.3 mg/L
Based on:
DOC
Initial conc.:
24 mg/L
Based on:
test mat.
Details on study design:
TEST CONDITIONS
- Test temperature: 21.6-22.9°C (mean: 22.3°C)

TEST SYSTEM
Vessel: approximately 5000 mL Woulff Flasks with standard ground joints
Test batches: 2 vessels without test item and with inoculum (blanks)
1 vessel containing control item and inoculum
2 vessels containing test item and inoculum
- Measuring equipment: Carbon analyser (Manufacturer Shimadzu)

SAMPLING
- Sampling frequency: 0,4, 7, 14, 18, 22, 25, 28 and 29 days
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (CO2 evolution)
Value:
0
Sampling time:
0 d
Parameter:
% degradation (CO2 evolution)
Value:
2
Sampling time:
4 d
Parameter:
% degradation (CO2 evolution)
Value:
1
Sampling time:
14 d
Parameter:
% degradation (CO2 evolution)
Value:
41
Sampling time:
22 d
Parameter:
% degradation (CO2 evolution)
Value:
92
Sampling time:
29 d
Details on results:
Kinetic of test substance (in %):
= 2 after 4 day(s)
= 4 after 18 day(s)
= 92 after 29 day(s)

BOD5 / COD results

Results with reference substance:
Kinetic of control substance (in %):
= 70 after 4 day(s)
= 90 after 18 day(s)
= 97 after 29 day(s)

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable