2,4-Imidazolidinedione, 5-(phenylmethylene)-, (5Z)-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(5Z)-5-(phenylmethylidene)imidazolidine-2,4-dione

EC Number:

700-728-5

Cas Number:

74805-60-0

Molecular formula:

C10H8N2O2

IUPAC Name:

(5Z)-5-(phenylmethylidene)imidazolidine-2,4-dione

Test material form:

solid: particulate/powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Suspended matter: 26.7 mg/L
- Dry matter: 4012 mg/L
- Colony forming units (CFU): 187 * 10^4 mL

Duration of test (contact time):

29 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

15.3 mg/L

Based on:

DOC

Initial test substance concentration 2

Initial conc.:

24 mg/L

Based on:

test mat.

Details on study design:

TEST CONDITIONS
- Test temperature: 21.6-22.9°C (mean: 22.3°C)

TEST SYSTEM
Vessel: approximately 5000 mL Woulff Flasks with standard ground joints
Test batches: 2 vessels without test item and with inoculum (blanks)
1 vessel containing control item and inoculum
2 vessels containing test item and inoculum
- Measuring equipment: Carbon analyser (Manufacturer Shimadzu)

SAMPLING
- Sampling frequency: 0,4, 7, 14, 18, 22, 25, 28 and 29 days

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

0 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

2

Sampling time:

4 d

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

1

Sampling time:

14 d

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

41

Sampling time:

22 d

% Degradation 5

Parameter:

% degradation (CO2 evolution)

Value:

92

Sampling time:

29 d

Details on results:

Kinetic of test substance (in %):
= 2 after 4 day(s)
= 4 after 18 day(s)
= 92 after 29 day(s)

BOD5 / COD results

Results with reference substance:

Kinetic of control substance (in %):
= 70 after 4 day(s)
= 90 after 18 day(s)
= 97 after 29 day(s)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable