Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 243-053-7 | CAS number: 19430-93-4
Endpoint summary
Administrative data
Description of key information
Additional information
Acute toxicity studies on fish, daphnia and algae to protocols equivalent or similar to OECD Guideline studies were conducted to GLP standards on PFBE (3,3,4,4,5,5,6,6,6-nonafluorohexene).
Fish
The objective of the study was the assessment of the acute toxicity of PFBE (3,3,4,4,5,5,6,6,6-nonafluorohexene) on the zebra fish, Danio rerio. Due to the low water solubility and high volatility of the test substance, the study was performed using water accommodated fractions (WAF) of PFBE as the test medium, as recommended for ecotoxicological testing of petroleum products by CONCAWE 1992 and for aquatic toxicity testing of lubricants by ASTM (standard protocol 06081-97). These protocols are designed to comply with the general principles outlined in the respective OECD guidelines.
The acute mortality of zebra fish under semi-static conditions was assessed after 24, 48, 72 and 96 hours. The validity criteria of the OECD Test Guideline 203 were fulfilled under the conditions of the modified procedures.
A concentration-effect relationship could not be established. The highest loading rate of 1000 mg/L PFBE (3,3,4,4,5,5,6,6,6-nonafluorohexene) revealed no toxicity different from controls.
Therefore, in the acute toxicity test with Danio redo (zebra fish), the effect values are as follows:
NOEC >/= 1000 mg/L (loading rate)
LOEC >/= 1000 mg/L (loading rate)
EC 50 > 1000 mg/L (loading rate)
NOEC >/= 1.86 mg/L (measured concentration)
LOEC > 1.86 mg/L (measured concentration)
EC 50 > 1.86 mg/L (measured concentration)
Daphnia
The study was performed to evaluate the effects of PFBE (3,3,4,4,5,5,6,6,6-nonafluorohexene) on the mobility of the freshwater crustacean, Daphnia magna, after a exposure times of 24 and 48 h. The objective of the study was the assessment of the acute toxicity of PFBE to Daphnia magna. Due to the low water solubility and high volatility of the test substance, the study was performed using water accommodated fractions (WAF) of PFBE as the test medium, as recommended for ecotoxicological testing of petroleum products by CONCAWE 1992 and for aquatic toxicity testing of lubricants by ASTM (standard protocol 06081-97). These protocols are designed to comply with the general principles outlined in the respective OECD guidelines. The mobility of the freshwater crustacean, Daphnia magna, under static conditions was assessed after 24 and 48 hours. The validity criteria of the OECD Test Guideline 202 are fulfilled under the conditions of the modified procedures.
Summary of the test results
Up to the highest nominal loading rate of 1000 mg/l no signifcant immobilisation was observed after 24 h and 48 h compared to controls.
NOEC >/=1000 mg/L (loading rate)
LOEC > 1000 mg/L (loading rate)
EC50 > 1000mg/L (loading rate)
NOEC >/= 1.0 mg/L (measured concentration)
LOEC > 1.0 mg/L (measured concentration)
EC50 > 1.0 mg/L (measured concentration)
Algae
The objective of the study was the assessment of the acute toxicity of PFBE on the green alga, Selenastrum capricornutum. Due to the low water solubility and high volatility of the test substance, the study was performed using water accommodated fractions (WAF) of PFBE as the test medium, as recommended for ecotoxicological testing of petroleum products by CONCAWE 1992 and for aquatic toxicity testing of lubricants by ASTM (standard protocol 06081-97). These protocols are designed to comply with the general principles outlined in the respective OECD guidelines.
The growth of the algae under static conditions was assessed after 24, 48, and 72 hours. The validity criteria of the OECD Test Guideline 201 are fulfilled under the conditions of the modified procedures.
Summary of the Algae test results
Inhibition of growth
Up to the highest nominal loading rate of 1000 mg/L PFBE (3,3,4,4,5,5,6,6,6-nonafluorohexene), no significant inhibition of growth (biomass) and growth rate was observed after 72 h compared to controls.
NOEC >/= 1000 mg/L (loading rate)
LOEC > 1000 mg/L (loading rate )
EC50 > 1000 mg/L (loading rate)
NOEC >/= 1.52 mg/L (lmeasured conc)
LOEC > 1.52 mg/L (measured conc )
EC50 > 1.52 mg/L (measured conc)
Promotion of growth
A promotion of growth (biomass) of 36.5 % was observed at the highest loading rate of 1000 mg/L after 72 h.
Conclusion of aquatic toxicity testing
No toxic effects were seen in fish, daphnia and algea in acute aquatic toxicity tests on PFBE (3,3,4,4,5,5,6,6,6-nonafluorohexene)
up to the maximum loading rate of 1000mg/L.Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
