4'-aminoazobenzene-4-sulphonic acid

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of test chemical

Justification for type of information:

Experimental data of various test chemical is from handbook

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

WoE report is prepared based on toxicity to bacteria study:
2nd and 3rd

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

other: Bacteria

Details on inoculum:

Unadapted biological waste water treatment plants

Test type:

not specified

Water media type:

other: Sewage water

Total exposure duration:

24 h

Details on test conditions:

3. Details on test conditions
TEST SYSTEM
- Test vessel: Fermentation tube

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: 2nd study

Dose descriptor:

EC0

Effect conc.:

> 2 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: 3rd study

Validity criteria fulfilled:

not specified

Conclusions:

2. Based on the growth rate inhibition of bacteria after exposure of 24 hrs with the test chemical, no effect were observed on the growth of bacteria. The EC0 was determine at the 500 mg/l.
3. Based on the growth rate inhibition of bacteria after exposure with test chemical, the EC0 was determine at > 2500 mg/l.
Thus based on the overall studies for the test chemical, chemical consider to be nontoxic and the toxicity ranges from the concentration of 500 - 2500 mg/l.

Executive summary:

Various studies available for the test chemical were reviewed to determine the toxic nature of test chemical on the growth and other activity of microorganisms. The studies are as mentioned below:

Aim of this study was to determine the effect of test chemical on the growth of bacteria. Unadapted biological waste water treatment plants were used. Test conducted for 24 hrs. Based on the growth rate inhibition of bacteria after exposure of 24 hrs with the test chemical, no effect were observed on the growth of bacteria. The EC0 was determine at the 500 mg/l. based on the EC0 chemical can be consider to be nontoxic to the growth of microorganisms.  

Above study was supported by the second study from peer reviewed journal. Study was conducted to determine the level of effect of test chemical on the growth of bacteria. Based on the growth rate inhibition of bacteria after exposure with test chemical, the EC0 was determine at > 2500 mg/l. As no effect were observed on the growth of bacteria chemical consider to be nontoxic.

Thus based on the overall studies for the test chemical, chemical consider to be nontoxic and the toxicity ranges from the concentration of 500 - 2500 mg/l.

Description of key information

2. Based on the growth rate inhibition of bacteria after exposure of 24 hrs with the test chemical, no effect were observed on the growth of bacteria. The EC0 was determine at the 500 mg/l.  

3. Based on the growth rate inhibition of bacteria after exposure with test chemical, the EC0 was determine at > 2500 mg/l.

Thus based on the overall studies for the test chemical, chemical consider to be nontoxic and the toxicity ranges from the concentration of 500 - 2500 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

500 mg/L

Additional information

Various studies available for the test chemical and structually and functionally similar read across chemical were reviewed to determine the toxic nature of test chemical on the growth and other activity of microorganisms. The studies are as mentioned below:

 

Aim of this study was to determine the effect of test chemical on the growth of bacteria. Unadapted biological waste water treatment plants were used. Test conducted for 24 hrs. Based on the growth rate inhibition of bacteria after exposure of 24 hrs with the test chemical, no effect were observed on the growth of bacteria. The EC0 was determine at the 500 mg/l. based on the EC0 chemical can be consider to be nontoxic to the growth of microorganisms.  

 

Above study was supported by the second study from peer reviewed journal. Study was conducted to determine the level of effect of test chemical on the growth of bacteria. Based on the growth rate inhibition of bacteria after exposure with test chemical, the EC0 was determine at > 2500 mg/l. As no effect were observed on the growth of bacteria chemical consider to be nontoxic.

 

Thus based on the overall studies for the test chemical, chemical consider to be nontoxic and the toxicity ranges from the concentration of 500 - 2500 mg/l.