Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

96 h LC50: 18 mg/L

Key value for chemical safety assessment

LC50 for freshwater fish:
18 mg/L

Additional information

In the Klimisch 1 GLP study from Kimmel (2013) the acute toxicity of Butanedioic acid, sulfo-, 4-C12-14-alkyl esters, disodium salts to zebra fish (Danio rerio) was determined in a 96‑hour semi-static test with daily test medium renewal according to the EU Commission Directive 92/69/, Part C.1, the Commission Regulation (EC) No 440/2008, Part C.1 and the OECD Guideline for Testing of Chemicals No. 203 (1992). The following nominal concentrations of Butanedioic acid, sulfo-, 4-C12-14-alkyl esters, disodium salts were tested:1.0, 3.2, 10, 32 and 100 mg test item/L. Additionally, a control (test water without test item) was tested in parallel. In the analyzed biologically relevant test media samples (10 to 100 mg/L) from the start and end of the first and last test medium renewal periods the measured test item concentrations ranged between 100 and 110% of the nominal values. Consequently, the test water renewal ensured nearly constant concentration of the test item during the test and the reported biological results were related to the nominal test item concentration. In the control and at the test concentrations up to and including 10 mg/L, all fish survived until the end of the test and no visible abnormalities were observed in the test fish. At the next higher test concentration of 32 mg/L, all test fish showed one or several visible abnormalities. After 48 hours test duration, all seven test fish died at this test concentration. At the highest test concentration of 100 mg/L, all fish were dead already after 24 hours of test duration. In the control and at the test concentrations up to and including 10 mg/L, all fish survived until the end of the test and no visible abnormalities were observed in the test fish. At the next higher test concentration of 32 mg/L, all test fish showed one or several visible abnormalities. After 48 hours test duration, all seven test fish died at this test concentration.

The 96‑hour LC50of Butanedioic acid, sulfo-, 4-C12-14-alkyl esters, disodium salts was calculated to be 18 mg/L with a 95% confidence interval of 10-32 mg/L. The 96‑hour LC100was 32 mg/L.

 

The results of this study are considered relevant and reliable for the risk assessment.