3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid

Surface tension

Administrative data

Endpoint:

surface tension

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 01, 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study (OECD109) with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 115 (Surface Tension of Aqueous Solutions)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method A.5 (Surface Tension)

Deviations:

no

GLP compliance:

no

Type of method:

OECD harmonised ring method

Test material

Test material information

Constituent 1

Reference substance name:

3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid

EC Number:

213-485-0

EC Name:

3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid

Cas Number:

957-68-6

Molecular formula:

C10H12N2O5S

IUPAC Name:

(6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Name: 7-Aminocefalosporansäure (7-ACA)
Lot no.: B146988

Results and discussion

Surface tension

Surface tension:

65 mN/m

Temp.:

20 °C

Conc.:

554.22 mg/L

Any other information on results incl. tables

The results of two measurements are summarized inTable1.

Table1: Measured surface tension values

Value No.	1^stmeasurements s[mN/m]	2^ndmeasurement s[mN/m]
1	64.71	65.21
2	64.71	65.22
3	64.70	65.21
4	64.69	65.20
5	64.69	65.20
6	64.69	65.20
Mean value of the individual values	64.70	65.21
Total mean value	64.95

The test item has a mean surface tension of 65.0 mN/m. Therefore the test item is not to be regarded as surface active.

Applicant's summary and conclusion

Conclusions:

According to OECD Test Guideline 115 and EC No. 440/2008 Part A. Method A.5. the surface tension at 20 °C of test item 7-Aminocephalosporanic acid was determined to be:
 = 65.0 mN/m
The test item is not to be regarded as surface active.

Executive summary:

Before the measurement the apparatus was cleaned very carefully. The measurement vessel was cleaned with double-distilled water and subsequently dried. The ring was rinsed with water to remove any water soluble substances, washed in double-distilled water and briefly heated over a flame.

The tensiometer was calibrated using a mass of 550 mg to obtain the calibration factors_afor multiplying all the instrument readings, which was done automatically.

For two tests solutions of the test item were prepared in distilled water. To obtain a concentration of 90 % of the saturation solubility 4 mL distilled water were added to 36 mL of a test solution (c = 615.8 mg/L) of the water solubility test of project CSL-12-0645.05. The concentration of the measured 90 % solutions was 554.22 mg/L.

The 90 % solution to be measured was transferred to the measurement vessel and the measurement vessel was placed on the table of the test apparatus. The temperature was adjusted to 20 °C.

The measurement vessel was raised automatically until the ring was immersed below the surface of the solution. Subsequently, the table top was lowered automatically to detach the ring from the surface until the maximum force was reached. The force was read on the tensiometer. The liquid layer attached to the ring was not separated from the ring.

After completing the first measurement, measurements were repeated until a constant surface tension value was reached.

The obtained surface tension values, presented by the tensiometer, are corrected values. The “really” measured values were first multiplied with the calibration factor and corrected afterwards by another correction factor depending on the ring dimensions, the density of the liquid and its surface tension. The corrections were automatically performed by the Tensiometer according to Zuidema & Waters.