Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
not specified
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
purity: 99+-2% (m/m)

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
source: waster water treatment plant Frankfurt-Sindlingen

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
no

Test conditions

Test temperature:
19.3 - 20.6 °C
pH:
7.0 - 7.1
Nominal and measured concentrations:
500 mg/l and 1000 mg/L nominal
Details on test conditions:
For each concentration, two replicates were performed (A) and (B)
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
Concentration respiration
mg/L inhibition %
1000 - 2.0
1000 - 2.0
500 7.4
500 7.4
Results with reference substance (positive control):
3,5-Dichlorophenol
Concentration respiration
mg/L inhibition %
5 22.8
20 66.9
30 71.3

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of N-(4-carbamoylphenyl)-4-nitrobenzamide for bacteria toxicity is > 1000 mg/L
Executive summary:

N-(4-carbamoylphenyl)-4-nitrobenzamide was tested for toxicity to bacteria according to OECD 209 (Activated Sludge, Respiration Inhibition Test). Two concentrations (500 mg/L and 1000 mg/L) were used in replicates. The EC50 of N-(4-carbamoylphenyl)-4-nitrobenzamide for bacteria toxicity is > 1000 mg/L.