N-[4-(aminocarbonyl)phenyl]-4-nitrobenzamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-[4-(aminocarbonyl)phenyl]-4-nitrobenzamide

EC Number:

298-791-2

EC Name:

N-[4-(aminocarbonyl)phenyl]-4-nitrobenzamide

Cas Number:

93839-21-5

Molecular formula:

C14H11N3O4

IUPAC Name:

N-(4-carbamoylphenyl)-4-nitrobenzamide

Details on test material:

purity: 99,7 %
pH in water about 8.3 (20°C)
Water solubility < 0.1 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

The test article was suspended with 0.095 ml Tween 80 to support dissolving of the substance

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrabärbling, Brachydanio rerio
- Strain: Hamilton-Buchanan
- Source: West Aquarium, bad Lauterberg
- Length at study initiation: 2.8 - 3.4 cm
- Feeding during test: twice a day; ad libitum
- Food type: Tetra Standard Mix, Tetra Werke Melle

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Food and Feeding frequency: same as test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Test temperature:

21.4 - 22.0 °C

pH:

7.4 - 8.2

Dissolved oxygen:

5.2 - 8.7 mg/L

Nominal and measured concentrations:

nominal: 500mg/L; measured: 197 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Open glass-vessel (30cm x 22cm x 24 cm), calibrated for 10 liters
- Aeration: none; except vessel wit 197 (500) mg/L after 72 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates):2 one with, one without Tween 80

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

197 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Due to LD50>197 mg/kg p-Nitrobenzoylaminobenzamid has not to be classified regarding its toxicity to fish

Executive summary:

p-Nitrobenzoylaminobenzamid TTR was tested in a concentration of 500 mg/L nominal (= 197 mg/L measured concnetration) for its toxicity to fish.

Control groups were pure water and water with Tween 80. In the tested concentration no lethality occured. Therefore, LD50 > 197 mg/L.