Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
purity: 99,7 %
pH in water about 8.3 (20°C)
Water solubility < 0.1 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
The test article was suspended with 0.095 ml Tween 80 to support dissolving of the substance

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrabärbling, Brachydanio rerio
- Strain: Hamilton-Buchanan
- Source: West Aquarium, bad Lauterberg
- Length at study initiation: 2.8 - 3.4 cm
- Feeding during test: twice a day; ad libitum
- Food type: Tetra Standard Mix, Tetra Werke Melle

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Food and Feeding frequency: same as test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
no

Test conditions

Test temperature:
21.4 - 22.0 °C
pH:
7.4 - 8.2
Dissolved oxygen:
5.2 - 8.7 mg/L
Nominal and measured concentrations:
nominal: 500mg/L; measured: 197 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Open glass-vessel (30cm x 22cm x 24 cm), calibrated for 10 liters
- Aeration: none; except vessel wit 197 (500) mg/L after 72 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates):2 one with, one without Tween 80

Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
197 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Due to LD50>197 mg/kg p-Nitrobenzoylaminobenzamid has not to be classified regarding its toxicity to fish
Executive summary:

p-Nitrobenzoylaminobenzamid TTR was tested in a concentration of 500 mg/L nominal (= 197 mg/L measured concnetration) for its toxicity to fish.

Control groups were pure water and water with Tween 80. In the tested concentration no lethality occured. Therefore, LD50 > 197 mg/L.