Trisodium bis[2-[[2,4-dihydroxy-3-[(2-methyl-4-sulphophenyl)azo]phenyl]azo]benzoato(3-)]chromate(3-)

Long-term toxicity to aquatic invertebrates

Description of key information

The nominal concentrations tested were 1.0, 3.2, 10.0, 32.0 and 100 mg test article/L and a control without any additions.
 
The test results:
 
Highest concentration tested without toxic effects (NOEC): 10 mg test article/L
Lowest concentrations tested with toxic effects (LOEC): 32.0 mg test article/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

10 mg/L

Additional information

The influence of the test item on reproduction and survival rate of daphnia magna was investigated in a 21 days semistatic test according to the OECD Guideline for testing of Chemicals, Section 2, No. 202, adopted 4 April 84.

 

The test was performed in a compliance with the Good Laboratory practice Regulations.

 

The nominal concentrations tested were 1.0, 3.2, 10.0, 32.0 and 100 mg test article/L and a control without any additions.

 

The test results:

 

Highest concentration tested without toxic effects (NOEC): 10 mg test article/L

Lowest concentrations tested with toxic effects (LOEC): 32.0 mg test article/L

 

In the concentration of 10 mg test article/L the analysed concentrations in the freshly prepared test media amounted in the mean to 82.4 % of the nominal value. In the freshly prepared test media of the concentration of 100 mg/L on day 0 only 54.4 % of the nominal value was measured, but in the lower concentration of 10 mg/L, prepared in sequential dilutions out of the higher concentrations, the measured concentration amounted to 84.7 % at that date. On the next analytical date 90.5 % of the nominal value of the concentration of 100 mg test article/L was determined. Since in the samples of the stock solutions always concentrations between 81.3 and 88.7 % of the nominal values were measured and the test article was sufficiently stable under test conditions, all reported results are related to the nominal concentrations of the test article.