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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1963
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well documented study result, which meets basic scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1963
Report date:
1963

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
A Choline chloride solution (8 % in aqua dest.) was administered to mice via intraperitoneal injection.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Choline chloride
EC Number:
200-655-4
EC Name:
Choline chloride
Cas Number:
67-48-1
Molecular formula:
C5H14NO.Cl
IUPAC Name:
2-hydroxy-N,N,N-trimethylethanaminium chloride
Test material form:
other: liquid
Details on test material:
Name of test material (as cited in report): Choline chloride (70 % in aqua dest.)
Name of Test material: Trimethyl-beta-hydroxyethyl-ammoniumhydrochlorid

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
no data available

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: aqua dest.
Details on exposure:
The substance was administered via intraperitoneal injection to mice.
Doses:
ca 0.35 mL of a 70% solution in aqua dest., applied as a 8% solution
No. of animals per sex per dose:
no details given
Control animals:
not specified
Details on study design:
no specific information available

Results and discussion

Effect levelsopen allclose all
Sex:
not specified
Dose descriptor:
other: approximate LD50
Effect level:
ca. 380 mg/kg bw
Based on:
test mat.
Remarks:
70% solution
Sex:
not specified
Dose descriptor:
LD50
Effect level:
ca. 266 mg/kg bw
Based on:
act. ingr.
Remarks:
Choline Chloride
Remarks on result:
other: re-calculated for the pure compound
Mortality:
none reported
Clinical signs:
restlessness, staggering, cramps and laboured breathing
Body weight:
not reported
Gross pathology:
no significant findings

Applicant's summary and conclusion

Conclusions:
The study was considered to be of high quality (reliability Klimisch 2). The substance did not show a high toxicity after a single intraperitoneal injection to mice. The LD50 was identified to be ca. 266 mg/kg bw (corresponding to 100 % Choline chloride).
Executive summary:

The acute toxicity of Choline chloride after intraperitoneal injection was investigated in mice (BASF, 1963). The mice received doses of ca. 0.35 mL of a 70 % Choline chloride solution (applied as a 8 % solution in water). No mortality was reported. The main clinical symptoms of intoxications were restlessness, staggering, cramps and laboured respiration. The intraperitoneal toxicity with an approximative LD50 of ca. 266 mg/kg bw of 100 % Choline chloride was found.

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