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Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Remarks:
Growth inhibition test according to Brinkmann and Kühn
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study without information about GLP compliance. No detailled documentation available about the test procedure.
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
no
Remarks:
according to the OECD SIDS report
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material: No surrogate or analogue material was used.
Analytical monitoring:
not specified
Details on sampling:
No details available.
Details on test solutions:
No details available.
Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Strain: DSM 50026
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 h
Post exposure observation period:
No post exposure observation period described.
Hardness:
No details available.
Test temperature:
297 +/- 1 K (22.85 - 24.85 °C)
pH:
- Control: 7.2
- 50 mg/L: 7.2 (start), 5.0 (end)
- 100 mg/L: 7.2 (start), 5.0 (end)
- 150 mg/L: 7.2 (start), 4.0 (end)
- 200 mg/L: 7.2 (start), 4.0 (end)
- 250 mg/L: 7.2 (start), 4.1 (end)
- 300 mg/L: 7.2 (start), 4.0 (end)
Dissolved oxygen:
No details available.
Salinity:
Not applicable.
Nominal and measured concentrations:
0, 50, 100, 150, 200, 250 and 300 mg/L
Details on test conditions:
- Test vessel: Erlenmeyer with nominal value of 300 mL
- Shaking: yes, at 150 rpm
- Medium: according to the national guideline DIN 38412, part 8
- Stock solution of the test substance: 1250 mg/L
- Growth period: 7 +/- 1 h
- Test preparation: The total volume was 10 mL. 0.5 mL of nutrient broth concentrate I and II were used (each: 20-fold). 1 mL bacteria suspension was adjusted to 10 FTU using NaCl solution
- Replicates: 4 inoculated samples and 1 unioculated sample were measured per concentration
- Wavelength for measurements: 436 nm
Reference substance (positive control):
no
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
112.9 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
132.8 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
other: EC90
Effect conc.:
278.2 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
growth inhibition
Details on results:
No further details available.
Results with reference substance (positive control):
Not applicable.
Reported statistics and error estimates:
A standard deviation was calculated from the individual values.

Table 1. Extinktion Measurement after 17 h at 436 nm

Concentration

Inoculated

Noninoculated

Difference

% of the control

Control

 

0.405

 

 

50 mg/L

0.425

0.000

0.425

104.81

100 mg/L

0.471

0.000

0.471

116.10

150 mg/L

0.062

0.000

0.062

15.24

200 mg/L

0.066

0.000

0.066

16.29

250 mg/L

0.061

0.000

0.061

15.05

300 mg/L

0.025

0.000

0.025

6.11

Validity criteria fulfilled:
yes
Remarks:
Basic validity criteria were fulfilled. National guideline study.
Conclusions:
Only basic information given. The test substance was found to be non-toxic towards Pseudomonas putida (EC50(17h): 132.80 mg/L). The study is classified as reliable with restrictions (Klimisch 2).
Executive summary:

The toxicity of Choline chloride (dissolved in water) towards Pseudomonas putida was investigated according to the German guideline DIN 38412 Part 8 (BASF AG, 2003). The experiment was conducted at 297 +/- 1 K (22.85 - 24.85 °C) for a time duration of 17 h. The test concentrations were 0 (control), 50, 100, 150, 200, 250 and 300 mg/L. The following results were obtained: EC10(17h): 112.90 mg/L, EC50(17h): 132.80 mg/L and EC90(17h): 278.20 mg/L, respectively.

Description of key information

Pseudomonas putida_DIN 38412 Part 8: EC10(17h): 112.90 mg/L, EC50(17h): 132.80 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:
132.8 mg/L
EC10 or NOEC for microorganisms:
112.9 mg/L

Additional information

The toxicity of Choline chloride (dissolved in water) towards Pseudomonas putida was investigated according to the German guideline DIN 38412 Part 8 (BASF AG, 2003). The experiment was conducted at 297 +/- 1 K (22.85 - 24.85 °C) for 17 h. The test concentrations were 0 (control), 50, 100, 150, 200, 250 and 300 mg/L. The following results were obtained: EC10(17h): 112.90 mg/L, EC50(17h): 132.80 mg/L and EC90(17h): 278.20 mg/L, respectively. Hence, choline chloride is non-toxic to Pseudomonas putida.

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