4-hydroxybutyl acrylate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 Dec 2004 to 16 Dec 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Version / remarks:

1986 (E) (Method B)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Name of test material (as cited in study report): Butanediolmonoacrylate
- Batch number: 010078 EDA 0
- Aggregate state: liquid
- Analytical purity: 99.3 corrected peak area-%
- Homogeneity: homogeneous
- Expiry date: May 2005
- Stability: guaranteed by sponsor

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Source: Activated sludge from laboratory wastewater plant treating municipal sewage.
- Concentration of dry substance: 1 g/L.
- Preparation of the inoculum: inoculum was washed and brought to a concentration of 5 g/L dry substance and aerated over night. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test temperature:

20 ± 2 °C

pH:

- pH value at test start (after correction): 7.1 - 7.2
- pH value after 30 min: 7.3 - 7.5

Dissolved oxygen:

- During aeration: >2.5 mg/L
- Immediately before measurement: > 6.5 mg/L

Nominal and measured concentrations:

- Nominal test concentrations: 0 (control), 100, 248, 496, and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: erlenmeyer-vessel
- Test volume: 250 mL

TEST MEDIUM / WATER PARAMETERS
- Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium

EFFECT PARAMETERS MEASURED: oxygen consumption
- Duration of the measurement of oxygen : 8 - 10 min

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

ca. 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

30 min

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

Inhibition of the respiration rate was 10% in the 100 mg/L dose group and increased in a dose-dependent manner up to 48% inhibition at the dose group of 1000 mg/L. For an overview of the results, see 'Any other information on results incl. tables'.

Results with reference substance (positive control):

The 30-min EC50 of the reference substance was determined to be ca. 9 mg/L.

Table: Respiration rate after 30 min of incubation.

Nominal Conc. [mg/l]	O2 consumption rate [mg O2/L*h]	Change in O2 consumption rate [%]
0 (control)	21*	-
100	19	10
248	18	14
496	15	29
1000	11	48

* mean value of blank control (3 replicates)

 

Validity criteria fulfilled:

yes

Remarks:

see 'Any other information on materials and methods incl. tables'.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): Butanediolmonoacrylate

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Source: Activated sludge from a laboratory waste water treatment plant treating municiple waste water.
- Initial biomass concentration: Concentration of activated sludge: 1 g/L dry matter

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Nominal and measured concentrations:

- Nominal test concentrations: 0 (control), 100 and 1000 mg/L

Reference substance (positive control):

not specified

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

At 100 mg/L, 14% of respiration inhibition was observed. No inhibition of respiration was observed at 1000 mg/L, however. See 'Any other information on results incl. tables'.

Table: Respiration rate after 30 min of incubation.

Nominal Conc. [mg/l]	DOC conc. [mg/L]	Respiration rate [mg/L x h]	Respiration inhibition [%]
0	-	22	-
100	58.1	25	14
1000	583.1	22	-

 

 

Reference 3

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

without detailed documentation.

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): Butanediolmonoacrylate

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

- Concentration of the stock solution: 12500 mg/L.

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Strain: DSM 50026

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

7 h

Test temperature:

297 ± 1 K

Nominal and measured concentrations:

- Nominal test concentrations: 0 (control), 78.125, 156.25, 312.5, 625, 1250, 2500, 5000, and 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyers (300 mL)
- Used volume: 100 mL test media
- Shaking conditions: yes

TEST MEDIUM / WATER PARAMETERS
- Medium: AK-medium according to DIN 38412 part 8
- Stock solution: concentration 12500 mg/L

TEST SOLUTION
- 0.5 mL nutrient solution I (20 fold)
- 0.5 mL nutrient solution II (20 fold)
- 1 mL bacteria suspension
- 8 mL test substance solution

Reference substance (positive control):

no

Duration:

7 h

Dose descriptor:

EC10

Effect conc.:

343.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

7 h

Dose descriptor:

EC50

Effect conc.:

842.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

7 h

Dose descriptor:

other: EC90

Effect conc.:

1 320.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Table: growth inhibition after 7 hours exposure.

Nominal Conc. [mg/l]	Growth inhibition in % of the control
78.125	108.64
156.25	100.3
312.5	92.1
625	71.01
1250	10.57
2500	0.44
5000	0.10
10000	0.10

Description of key information

The 30-min EC50 value is >1000 mg/L as observed in a respiration inhibition study with activated sludge treating domestic sewage.

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

Additional information

The toxicity of 4-hydroxyethyl acrylate to microorganisms was investigated in three different studies according to OECD guideline 209 guideline and DIN 38412 Part 8.

The study conducted most recent was in compliance with GLP regulations and performed according to OECD guidelines and therefore selected as key study. The toxicity of the substance to microorganisms was investigated in an activated sludge respiration inhibition study according to OECD TG 209 and in compliance with GLP criteria (BASF, 2005). In this study, non-adapted activated sludge treating domestic sewage was exposed to nominal test substance concentrations of 0 (control), 100, 248, 496, and 1000 mg/L for 30 minutes. Test concentrations were not analytically verified. The effects on microorganisms was determined by measuring total respiration rates and comparing these to an unexposed control. Inhibition of respiration rates was first observed from the lowest test concentration of 100 mg/L (10%). The effects increased concentration-dependently reaching 48% at the highest concentration tested. Based on these findings, the 30-min EC50 and EC20 values were determined at >1000 mg/L and ca. 320 mg/L, respectively.

In a supporting study, performed according to OECD 209, domestic activated sludge was exposed to nominal test concentrations of 0 (control), 100 and 1000 mg/L for 30 minutes (BASF, 1989). At 100 mg/L, the inhibition was determined to be 14%. No inhibition of respiration was measured at 1000 mg/L, however. The 30-min EC20 value was determined to be >1000 mg/L.

In the other study, bacteria (Pseudomonas putida) were exposed to nominal test substance concentrations of 0 (control), 78.125, 156.25, 312.5, 625, 1250, 2500, 5000, and 10000 mg/L for 7 hours (BASF, 1989). The inhibition of growth increased in a dose-dependent manner from 312.5 mg/L up to 10000 mg/L. The EC50 and EC10 values were determined to be 842.3 mg/L and 343.6 mg/L, respectively.