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Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed guideline study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
not specified
Qualifier:
equivalent or similar to guideline
Guideline:
other: draft ISO/DIS 10712 (1994)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitro-p-toluic acid
EC Number:
202-549-3
EC Name:
3-nitro-p-toluic acid
Cas Number:
96-98-0
Molecular formula:
C8H7NO4
IUPAC Name:
4-methyl-3-nitrobenzoic acid
Details on test material:
purity: 99.4 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Laboratory culture:
- Method of cultivation: analogous DIN 38 412 Teil 8 (1991)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
16 h

Test conditions

Test temperature:
21+-1 °C
pH:
7.3
Nominal and measured concentrations:
nominal: 500 mg/L and 1000 mg/L
Reference substance (positive control):
yes
Remarks:
2,4-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
16 h
Dose descriptor:
other: 0.3 % inhibition
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
16 h
Dose descriptor:
other: 19.6 % inhibition
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Results with reference substance (positive control):
2,4-Dichlorophenol after 16 h
30 mg/L : 26.2 % inhibition
90 mg/L: 97.6 % inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The substance is not toxic to Pseudomonas putida
Executive summary:

The substance was tested for toxicity for Pseudomonas putida according to DIN 38 412 Teil 8 (1991) by photometric determination of the cell density. 2,4 -Dichlorophenol was used as positive reference substance. After 16 hours the inhibition was 19.6 % with 1000 mg/L and 0.3 % with 500 mg/L. The EC 50 is greater than 1000 mg/L