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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitro-p-toluic acid
EC Number:
202-549-3
EC Name:
3-nitro-p-toluic acid
Cas Number:
96-98-0
Molecular formula:
C8H7NO4
IUPAC Name:
4-methyl-3-nitrobenzoic acid
Details on test material:
purity: 99.4 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebra fish; Brachydanio rerio (HAMILTON-BUCHANAN)
- Source: Corporate Toxicology (Hoechst AG)
- Age at study initiation: approximately two months
- Length at study initiation: 2.8 - 3.3 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test or not
- Type and amount of food: Tetra Min, Tetra Werke, Melle
- Feeding frequency: twice daily ad libitum

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
2.1 mmol Ca 2+ Mg 2+ / L
Test temperature:
21.6 - 22.2 °C
pH:
7.5 - 7.7
Dissolved oxygen:
7.6 - 9.5 mg/L
Nominal and measured concentrations:
nominal: 100 mg/L
measured (average): 99.1 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass, calibrated to 10 liters
- Material, size, headspace, fill volume: 30 cm x 22 cm x 24 cm; Hostalit Z
- Aeration: no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water, composition according to ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Photoperiod: 12 hours dark / 12 hours light
- Light intensity: 700 lux

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
0 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: control
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
No changes in appearance and behavior
No mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the test result the LC50 is greater than 100 mg/L concerning the acute toxicity to fish.
Executive summary:

The substance was tested for acute toxicity to fish according to OECD guideline 203. One concentration (100 mg/L) was tested over a period of 96 hours.

No lethality occurred and the fish showed no changes in appearance and behavior in comparison to the control group (0 mg/L).

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