cyclohexyl salicylate

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1985

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexyl Salicylate

IUPAC Name:

Cyclohexyl Salicylate

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.18 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Any other information on results incl. tables

pH was maintained between 7.4 and 7.7 during the study, O₂between 7.8 and 8.3 mg/L and temperature 20 to 21 °C. 100% mortality of Daphnids was observed at nominal test concentrations of 2 mg/L and above. At the 1.0 mg/L (nominal) test concentration effects were observed on the fecundity, the number of offspring per Parent Daphnid being significantly inferior to that of the Control. The NOEC (21-day) was 0.5 mg/L (nominal) / 0.18 mg/L (measured - extrapolated).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The long-term toxicity of CYCLOHEXYL SALICYLATE to Daphnids(Daphnia magna) was studied in reconstituted water over a period of 21-Days under semi-static renewal conditions. Test concentrations studied were 0.3, 0.5. 1, 2.0, 3.0, 5.0 and 10 mg/L (nominal) and a Control. Substance specific analyses showed that between 40 and 60% of the nominal test concentration was detected in Time 0 samples. Losses of CYCLOHEXYL SALICYLATE from the test system was up to 70% over a 24 hour period. It was suggested in the report that the initial analysed concentration was 50% of the nominal, and that the value was 25% of nominal in the aged medium prior to being changed. For example, at the nominal concentration of 0.5 mg/L, the initial analysed concentration would be 0.25 mg/L and the concentration in the aged medium 0.12 mg/L, suggesting an average measured exposure concentration of 0.18 mg/L. Mortality and fecundity were measured during the course of the experiment