Registration Dossier
Registration Dossier
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EC number: 247-384-8 | CAS number: 25973-55-1
Specific investigations: other studies
Administrative data
- Endpoint:
- endocrine system modulation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Reliable study report (not GLP)
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 003
Materials and methods
- Principles of method if other than guideline:
- 11 kinds of UV stabilizers used in food contact plastics were tested for their estrogenic activity by the yeast two-hybrid assay.
- GLP compliance:
- no
- Type of method:
- in vitro
- Endpoint addressed:
- other: estrogenic activity
Test material
- Reference substance name:
- 2-(2H-benzotriazol-2-yl)-4,6-ditertpentylphenol
- EC Number:
- 247-384-8
- EC Name:
- 2-(2H-benzotriazol-2-yl)-4,6-ditertpentylphenol
- Cas Number:
- 25973-55-1
- Molecular formula:
- C22H29N3O
- IUPAC Name:
- 2-(2H-benzotriazol-2-yl)-4,6-bis(1,1-dimethylpropyl)phenol
Constituent 1
Test animals
- Species:
- other: not applicable, in vitro assay
- Strain:
- other: not applicable
Administration / exposure
- Route of administration:
- other: not applicable
- Vehicle:
- other: DMSO
- Details on exposure:
- - The yeast cells were preincubated overnight at 30°C with vigorous shaking in a synthetic dropout (SD) medium free from tryptophan and leucine.
- test chemical solution was added and the mixture was incubated for 4 h at 30°C. - Duration of treatment / exposure:
- 4 h
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1 mM - 0.1 µM
Basis:
nominal conc.
Examinations
- Examinations:
- The results were evaluated based on relative activity, expressed as 10% relative effective concentration (REC 10), which is the concentration of the
test chemical showing 10% of the agonist activity of 1 E-6 M 17ß-Estradiol, the highest activity level of 17ß-Estradiol. - Positive control:
- 17ß-Estradiol
Results and discussion
- Details on results:
- REC 10 < 1.0 E -4
None of the tested Benzotriazole compounds - among these the test substance - showed estrogenic activity.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
