Methacrylic anhydride

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Method sufficiently described.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl methacrylate

EC Number:

201-297-1

EC Name:

Methyl methacrylate

Cas Number:

80-62-6

Molecular formula:

C5H8O2

IUPAC Name:

methyl methacrylate

Details on test material:

Purity: 99.8% MMA

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.55 - 2.8 kg

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

REMOVAL OF TEST SUBSTANCE
After the 24-h exposure, the cuffs were removed and the application sites were gently wiped with paper towels to remove the test substance. Despite this procedure, some of the test substance remained on the skin and fur staining them yellow. Subsequently animals were observed preening areas of treated skin.

Duration of exposure:

24 hrs

Doses:

0.2, 2.0 and 5.0 g/kg

No. of animals per sex per dose:

two males per dose

Control animals:

no

Details on study design:

Animals were observed 14 days post-dosing

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Mortality:

There were no deaths observed

Clinical signs:

other: There were no clincial signs observed

Gross pathology:

There were no gross necropsy changes observed

Other findings:

There was well defined to severe erythema with blanching, and moderate to severe edema with pocketing were observed at 24 hr at one or more dose levels. Skin irritation did not disappear at 14 d at 2 or 5 g/kg but did disappear at day 3 at 0.2 g/kg. Eschar was observed at day 2 at 2 and 5 g/kg but some eschar was observed to be sloughing off with new hair growth on the underlying skin at day at both dose levels. Dessication was observed after day 4 at all dose levels.