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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014
Report Date:
2014

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: viscous liquid
Details on test material:
- Name of test material (as cited in study report): BI 10773 TMS-Lactone
- Analytical purity: 96.6%
- Impurities (identity and concentrations): CD 6332 silylated 2.0 % / Unspecified impurity 1.4 %
- Purity test date: 04.2014
- Lot/batch No.: 0002
- Expiration date of the lot/batch:: 30 April 2014
- Storage condition of test material: between 2 - 8°C

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Daphnia magna STRAUS, Clone V,
- Source: German Federal Environment Agency (UBA)
- Age at study initiation (mean and range, SD): max. 24 h
- Method of breeding: Daphnia magna was bred as single culture (one daphnid per 100 mL) in Elendt
M4 medium. The pH-value of the aerated water was within a range of 6.0 – 9.0.
The dissolved oxygen was above 60 % saturation and the total hardness 140 -
268 mg/L (as CaCO3).
- Feeding during test: single cell green algae
(Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three
times a week.

Study design

Test type:
static
Water media type:
not specified
Total exposure duration:
48 h

Test conditions

Hardness:
14°dH ( 250mg/L as CaCo3)
Test temperature:
19.5 – 20.4 °C
pH:
7.67 – 7.90
Dissolved oxygen:
97 %
Nominal and measured concentrations:
Test concentrations: 0.100 mg/L - 10.0 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass beaker
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 100 mL filled with 50 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Justification for using less concentrations than requested by guideline: A flat dose-response relationship was observed in the range-finding test.
- Test concentrations: 0.100 mg/L - 10.0 mg/L
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.32 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.04 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Results with reference substance (positive control):
- Results with reference substance valid? Yes
The results indicate an EC50 (24 h) of the reference item potassium dichromate of
2.0 mg/L. Since the results fall within the historical data generated with the
reference item at the testing facility and are within the range of 0.6 - 2.1 mg/L recommended by the test guideline OECD 202, the daphnids were suitable for the determination of the toxicological effects of the test item.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
According to the results of the test, the EC50 (48 h) is 1.04 mg test item /L (nominal) corresponding to 0.175 mg test
item/L (actual). The EC0 (48 h) was observed at 0.320 mg test item /L (nominal) and 0.0425 mg test item/L (actual), the
EC100 (48 h) at 10.0 mg test item/L (nominal) corresponding to 4.00 mg test item/L (actual).